Changes

* Lack of the typical Initially subdued "horsey", "funky", "barnyardy" flavors due to the lack of ''Saccharomyces'' esters/phenols (see the [[Brettanomyces#Brettanomyces_Metabolism|Brettanomyces Metabolism]] page for more information). However, this is a generalization and some brewers have reported getting some "funkier" flavors out of some strains.

A lot of the conventional wisdom listed above regarding 100% ''Brettanomyces'' fermentations is anecdotal information in relation to Trois fermentations. As explained above, Trois is not actually ''Brettanomyces'', and so conventional wisdom regarding 100% ''Brettanomyces'' beers has been brought into question. One particular area of question is the conventional wisdom that ''Brettanomyces'' requires phenols from POF+ ''Saccharomyces'' strains in order to convert 4-vinyl phenols into 4-ethyl phenols.  There is surprisingly little data to back this idea up outside of the anecdotal information gathered from brewers fermenting with 100% Trois, which was once thought to be ''Brettanomyces'' <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1226859710675574/?comment_id=1226864927341719&reply_comment_id=1226949030666642&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with Lance Shaner on MTF. 02/05/2016.]</ref>. One controlled experiment by [[Brettanomyces_secondary_fermentation_experiment|Lance Shaner of Omega Yeast Labs and Richard Preiss of Escarpment Labs]] showed that the levels of 4-ethyl guaiacol and 4-ethyl phenol produced by ''Brettanomyces'' did not depend on the amount of their 4-vinyl precursors, suggesting that ''Brettanomyces'' is capable of producing 4EP and 4EG ''de novo'' (without being dependent on precursors produced by ''Saccharomyces''). In addition to this, the possibility that some yeast labs have ''Saccharomyces'' contamination issues in their ''Brettanomyces'' products complicates the issue. This is only one data point, however, and more data needs to be researched. A study<ref>[https://www.facebook.com/groups/MilkTheFunk/1285391944822350/=fb_ctreasearch1 Some new data on 100% Brett brux fermentation and sugar utilization]</ref> conducted by Caroline Tyrawa measured, amongst other things, the 4-Ethyl phenol in 100% Brettanomyces Bruxellensis. It shows clear levels of 4-Ethyl phenol in wort fermented by various strains of the before-mentioned yeast.