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Laboratory Techniques

1,536 bytes added, 06:47, 4 September 2017
Propagators
'''Justin Amaral's 4.5 BBL Propagator'''
The propagator below has a 14 gallon capacity, allowing a prop up of 4.5 BBL. This same set up can be used on any fermenter but is bested used on ones that can hold 5 PSI and up. The seal on the below fermenter can hold around 5 PSI but clamps are also used on the lid to ensure it can hold up to 10 PSI. It essentially acts as a large stir plate while allowing to trickle in either O2, NO2 or CO2 depending on what your propagating. Ideally you'd also use a non magnetic drive pump but its not essential.
 
Below is the draw up of the build out for this prop up tank. As you can see it uses quick disconnects but you can use tri-clover connects as well. It also uses a diffusion stone that connects into one of the ferrules allowing you to trickle in gases. The picture shows a diffusion stone directly into the fermenter but its since been adapted as shown in the other pictures below to allow the diffusion stone to be behind a valve so its only exposed when needed. It also uses a whirlpool connector that goes into the fermenter creating the whirlpool when the pump is on.
[[File:4.5bblpropup3.jpg]]
 
 
Below you can see the 4 way SS T valve at the top of the fermenter allowing for a blow off tube, sterile transferring of yeast(the yeast has to be in a container with a tri-clover valve for this), and the hose coming from the kettle.
[[File:4.5bblpropup2.jpg]]
 
 
Below is the valve set up. The top valve has the whirlpool connect, the middle has the diffusion stone behind the valve, and the bottom has an elbow with a valve.
[[File:4.5bblpropup.jpg]]
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