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updated Brett starter temperatures and growth rates
Although more experiments and probably needed, agitation is believed to be an important factor for any species of microbe (yeast and bacteria). Gentle stirring on a stir plate or orbital shaker, or frequent gentle manual agitation leads to faster growth and a higher number of organisms. Agitation keeps the microbes in solution. It also maximizes the microbes' access to nutrients and disperses waste evenly. In a non-agitated starter, the microbes are limited to the diffusion rate of nutrients, leading to a slower and more stressful growth <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1168024059892473/?comment_id=1174865305875015&reply_comment_id=1176092372418975&total_comments=1&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Conversation with Bryan of Sui Generis Blog about starters and agitation. 11/09/2015.]</ref>.
Maintaining a temperature of 70°-80°F/20°-26°C should be adequate for most strains, however 77°-86°F/25°-30°C results in faster growth <ref name="Trent_Preiss_Brett_Starters">[https://www.facebook.com/groups/MilkTheFunk/permalink/1334091429952401/?comment_id=1334157506612460&reply_comment_id=1334686406559570&comment_tracking=%7B%22tn%22%3A%22R0%22%7D Conversation with Mark Trent and Richard Preiss on optimal temperatures for Brett starters on MTF. 06/21/2016.]</ref>. ''Brettanomyces'' cell growth typically takes about 7-8 days to reach it's maximum growth <ref name="Yakobson_Propagation"></ref>, however some strains may grow at faster rates and finish in 3-4 days <ref>[[Brettanomyces_Propagation_Experiment]]</ref>. ThusWhen the starter turns a rich creamy color, each it should be done within a few hours after this visual indication occurs <ref name="Trent_Preiss_Brett_Starters"></ref>. Each step of a starter for Brett should be 7-8 days(or 3-4 days for faster growing strains).
For more information regarding aeration and agitation effects on ''Brettanomyces'' growth, see [[Brettanomyces_Propagation_Experiment|Mark Trent's Brettanomyces Propagation Experiment]].