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Long Major yeast labs will often freeze yeast in liquid nitrogen using cryopreservation, however this option is generally not practical for brewers <ref>[https://books.google.com/books?id=gcJQAwAAQBAJ&pg=PA181&lpg=PA181&dq=Yeast+supply%E2%80%94the+challenge+of+zero+defects&source=bl&ots=u-9uoIvsFo&sig=XnqR3179u4mC9LqWotMX3Fu7MbU&hl=en&sa=X&ved=0ahUKEwiX-bKyvM_JAhVH4WMKHQr9C1cQ6AEIHTAA#v=onepage&q=liquid%20nitrogen&f=false Brewing: New Technologies. Charlie Bamforth. 2006. Pg 170.]</ref>. For brewers, long term storage of ''Brettanomyces'' should be frozen with 10% glycerol, rather than agar plates, which . Agar plates have been observed anecdotally to reduce viability of ''Brettanomyces'' over time <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1083075848387295/?comment_id=1083272091701004&offset=0&total_comments=13&comment_tracking=%7B%22tn%22%3A%22R0%22%7D Conversation with Matt Humbard, Ritchie Preiss, and Jeff Melo on MTF. 6/4/2015.]</ref><ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1115768398451373/?comment_id=1115817201779826&offset=0&total_comments=34&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Conversation with Nick Impellitteri on MTF regarding storing Brett on agar plates. 7/24/2015.]</ref>. Slants can provide storage for a few months up to 2+ years, depending on the type of slant used. For instructions on how to make slants at home capable of storing any microbe for potentially 2+ years, [http://suigenerisbrewing.blogspot.com/2015/11/easy-home-yeast-banking-and-video.html see Bryan's video on Sui Generis Brewing (requires a pressure cooker)].
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===Storing Brett===
Regarding temperature, Chad Yakobson noted that after storing ''Brettanomyces'' in a refrigerated environment (we don't know how Chad was storing the ''Brettanomyces'' cultures when he observed this, for example on agar plates or slants or something else.), after 6 months the ''Brettanomyces'' would die. If ''Brettanomyces'' is stored cold, it will be very sluggish and slow to start fermentation. Non-pure cultures (such as beer bottle dregs with ''Brettanomyces'' in it) should be stored refrigerated. Making a starter is highly recommended if the ''Brettanomyces'' culture has been stored cold <ref>[http://youtu.be/AjVOzBtE27Y?t=43m Yakobson, Chad. Presentation at 2012 Music City Brew Off. At 43:00.]</ref>.