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→Detection Methods
Kiyomichi et al. (2023) developed a simple method to accurately detect ATHP, ETHP, and APY using gas chromatography-mass spectrometry with stir bar sorptive extraction (SBSE-GC–MS instrumentation). Thermal desorption and injection were performed using a Twister thermal desorption unit (TDU) and a Gerstel CIS 4 cooled injection system with a programmable temperature vaporization (PTV) inlet, installed on an Agilent 6890 gas chromatograph combined with an Agilent 5975 Mass Selective Detector (Agilent Technologies, Massy, France), equipped with a Gerstel MPS 2 autosampler (Gerstel, Mülheim an der Ruhr, Germany). An HP-5MS fused-silica capillary column (30 m × 0.25 mm, 0.25 µm, film thickness, SGE, Courtaboeuf, France) was used, with helium as carrier gas (Messer France S.A.S, Suresnes, France) at a constant pressure of 70 kPa, corresponding to an initial flow of 1.3 mL.min−1 <ref>[https://www.sciencedirect.com/science/article/abs/pii/S0308814623000705 Daiki Kiyomichi, Céline Franc, Pierre Moulis, Laurent Riquier, Patricia Ballestra, Stéphanie Marchand, Sophie Tempère, Gilles de Revel. Investigation into mousy off-flavor in wine using gas chromatography-mass spectrometry with stir bar sorptive extraction. Food Chemistry, Volume 411, 2023, 135454, ISSN 0308-8146, https://doi.org/10.1016/j.foodchem.2023.135454.]</ref>. See also [https://ives-technicalreviews.eu/article/view/7867 "Simultaneous assay of mousy off-flavor markers in wine," Ives Technical Review].
Liesel Krout, Lucille Benedict, Meg Hausman, and Zach Bodah from University of Southern Maine and Allagash Brewing Company have begun developing a new way to produce an ATHP flavor spike and detection method. See [https://digitalcommons.usm.maine.edu/cgi/viewcontent.cgi?article=1180&context=thinking_matters the poster] for more details. This method is still in development.
In 2024, UC Davis described their method for detecting THP in beer for the first time. The team used Liquid Chromatography Mass Spectrometry with Electrospray Ionization (LC-MS-ESI). Extraction of ATHP in the samples was performed using QuEChERS (quick, easy, cheap, effective, rugged, and safe) technique <ref>[https://www.sciencedirect.com/science/article/pii/S2215016124000979 Paulina Martusevice, Xueqi Li, Matt Hengel, Selina C. Wang, Glen Fox, Analysis of mousy off-flavor compound 2-Acetyl-tetrahydropyridine using Liquid Chromatography Mass Spectrometry with Electrospray Ionization in sour beer, MethodsX, Volume 12, 2024, 102643, ISSN 2215-0161, https://doi.org/10.1016/j.mex.2024.102643.]</ref>. See also [https://www.masterbrewerspodcast.com/326 MBAA podcast episode 326, "THP" with Paulina Streimikyte and Glen Fox].
The effectiveness of these methods in beer has not been reported to our knowledge (please report any research or anecdotes in the [https://www.facebook.com/groups/MilkTheFunk/ MTF Facebook group]).
==Forms of THP==