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Commonly known as lager yeast to brewers, this yeast is a hybrid of ''S. eubayanus'' and ''S. cerevisiae'' <ref name="wikipedia_cereisiae" />.

Separated into two main lineages, "Saaz" and "Frohberg". The two lineages are believed to have descended from different hybridisation events between ''S. eubayanus'' and ''S. cerevisiae''. Emil Christian Hansen was the first in the world to isolate a single pure strain of ''S. pastorianus'', leading to its first classification name, ''S. carlbergensis''. The type that Hansen isolated was a Saaz type, and it's believed that Saaz types are also prevalent in Bohemian / Czech ''S. pastorianus'' strains.  With recent whole genome sequencing data, it has been discovered that some strains of commercial yeast have been misidentified as either lager yeast or ale yeast. [http://sykesey.id.au/?p=20 ben Ben Sykes reported] that publicly available genetic data for an upcoming study indicates that WLP800, the Czech lager yeast from White Labs, is potentially ''S. cerevisiae'' (ale yeast). WLP029 German Ale/ Kölsch Yeast is potentially lager yeast. WY1187 Ringwood is potentially lager yeast. WLP838 Southern German Lager Yeast is potentially ale yeast.