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==General Methods and Uses==
The following information is specific to collecting microbes from commercial bottles of mixed fermentation sour beers or ''Brettanomyces'' beers. For instructions on collecting ''S. cerevisiae'' from clean beers, check out [https://www.jaysbrewing.com/2012/09/05/9-steps-to-culture-yeast-from-a-bottle/ this guide].
===Making a Starter or Not===
There are several things to take into consideration when deciding to make a starter or not for bottle dregs. Since ''Brettanomyces'' [[Brettanomyces#Primary_versus_Secondary_Fermentation|doesn't need many cells]] to have an impact unless it is being used for 100% ''Brettanomyces'' fermentation, a starter is not necessary if the bottle is young and the brewer only wants to use the ''Brettanomyces''. If the bottle is older (6+ months from bottling), it might be a good idea to make a starter just to make sure the ''Brettanomyces'' is still viable.