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Laboratory Techniques

503 bytes added, 06:29, 1 September 2017
Brettanomyces
====''Brettanomyces''====
A few different medias can be used to isolate ''Brettanomyces'' but DBDM media is commonly used. WLN and WLD with additions of cycloheximide can also be used.
'''DBDM Media Recipe'''
|-
| p-coumaric acid || 100 mg
|-
| Bromocresol Green || 22 mg
|-
| Agar || 20 grams
|-
| Distilled Water || Fill to 1000 ML
|}
 
'''WLD Media Recipe'''
{| class="wikitable"
|-
! Chemical !! Usage Amount
|-
| Cycloheximide || 4 grams
|-
| Yeast Extract || 4 grams
|-
| Pancreatic Digest of Casein(Pepton) || 5 grams
|-
| Dextrose || 50
|-
| Monopotassium Phosphate || .55 grams
|-
| Potassium Chloride || 425 mg
|-
| Calcium Chloride || 125 mg
|-
| Magnesium Sulfate || 125 mg
|-
| Ferric Chloride || 2.5 mg
|-
| Manganese Sulfate || 2.5 mg
|-
| Bromocresol Green || 22 mg
318
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