Changes

The addition of vitamins can have a positive impact on ''Brettanomyces'' growth. For example, while ''Brettanomyces'' does not need riboflavin (vitamin B2) or thiamine (vitamin B1) in order to grow, the presence of either or both of these two vitamins encourages ''Brettanomyces'' growth <ref>[http://onlinelibrary.wiley.com/doi/10.1002/jib.385/full The influence of thiamine and riboflavin on various spoilage microorganisms commonly found in beer. Barry Hucker, Melinda Christophersen, Frank Vriesekoop. 2017.]</ref>. Other vitamins such as p-aminobenzoic acid (PABA), folic acid (vitamin B9), nicotinic acid (vitamin B3), pantothenic acid (vitamin B5) are also not required for most strains of ''Brettanomyces'' to grow. The presence of alcohol can increase the dependence on vitamins for some strains of ''Brettanomyces'' to grow. For example, Myo-inositol (vitamin B8) and thiamine (vitamin B1) were required by two strains of ''B. bruxellensis'' when grown in 10% ethanol but not in 0% ethanol. Biotin (vitamin B7) is the one exception, and it was found that the lack of biotin inhibited the growth of some strains of ''B. bruxellensis''. Other studies contradict these previously mentioned findings, showing that thiamine was not required by the strains of ''B. bruxellensis'' tested, that pyridoxine was required, and biotin was not required. These discrepancies between scientific studies are probably due to the genetic differences between the strains selected, the growth media chosen by the scientists, and/or the growth conditions <ref>[http://www.mdpi.com/2311-5637/2/3/17 Use of Nutritional Requirements for Brettanomyces bruxellensis to Limit Infections in Wine. Nicolas H. von Cosmos and Charles G. Edwards. 2016.]</ref>.

==''Brettanomyces'' Metabolism==