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Some preliminary work has been done by Mark Trent and [[File:Brett viability temp storage.jpg|thumb|300px|[https://www.facebook.com/groups/MilkTheFunk/permalink/1115768398451373/ Richard Preiss to examine the effects 's results of storing ''Brettanomyces'' in MYPG and wort at refrigeration temperatures versus room different temperatures for 30 days. This work suggested that cold storage for as long as one 1 month is better than room temperature storage for the same time. Chad Yakobson also noted that after storing ''Brettanomyces'' in a refrigerated environment (we don't know how Chad was storing the ''Brettanomyces'' cultures when he observed this, for example on agar plates or slants or something else.), after 6 months the ''Brettanomyces'' would die. If ''Brettanomyces'' is stored cold, it will be very sluggish and slow to start fermentation. Non-pure cultures (such as beer bottle dregs with ''Brettanomyces'' in it) should be stored refrigerated. Making a starter is highly recommended if the ''Brettanomyces'' culture has been stored cold <ref>[http://youtu.be/AjVOzBtE27Y?t=43m Yakobson, Chad. Presentation at 2012 Music City Brew Off. At 43:00.]</ref>.]]

[[File:Brett viability temp storageMarkTrent.jpg|thumb|300px|[https://www.facebook.com/groups/MilkTheFunk/permalink/1115768398451373/ ?comment_id=1145139948847551&offset=0&total_comments=69&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Mark Trent repeated Richard Preiss's results of storing Brett ''Brettanomyces'' at different temperatures, and also in different mediums for 1 month.]]]

[[File:Brett Some preliminary work has been done by Mark Trent and Richard Preiss to examine the effects of storing ''Brettanomyces'' in MYPG and wort at refrigeration temperatures versus room temperatures for 30 days. This work suggested that cold storage for as long as one month is better than room temperature storage MarkTrentfor the same time. Chad Yakobson also noted that after storing ''Brettanomyces'' in a refrigerated environment (we don't know how Chad was storing the ''Brettanomyces'' cultures when he observed this, for example on agar plates or slants or something else.jpg|thumb|300px|), after 6 months the ''Brettanomyces'' would die. If ''Brettanomyces'' is stored cold, it will be very sluggish and slow to start fermentation. Non-pure cultures (such as beer bottle dregs with ''Brettanomyces'' in it) should be stored refrigerated. Making a starter is highly recommended if the ''Brettanomyces'' culture has been stored cold <ref>[httpshttp://wwwyoutu.facebook.com/groups/MilkTheFunk/permalink/1115768398451373be/AjVOzBtE27Y?comment_idt=1145139948847551&offset=0&total_comments=69&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Mark Trent repeated Richard Preiss's results of storing Brett 43m Yakobson, Chad. Presentation at different temperatures, and also in different mediums2012 Music City Brew Off. At 43:00.]]]</ref>.

Mark Trent repeated Richard Preiss's experiment, but tested different mediums (wort, liquid MYPG, and water). Two ''Brettanomyces'' isolates, one from Orval and one from SARA Bernice, were grown in 10 degree Plato wort and MYPG. The isolates are labeled on the chart as "Brett O (Orval)" and "Brett T (Tim Clifford)" respectively. After growth was complete, 10 mL of aliquots were aseptically transferred to 15 mL centrifuge tubes. In addition, the Orval isolate was grown on a MYPG plate and 3 single colonies for each treatment were transferred to 1 mL of sterile RO water in a 2 mL glass tube. Each treatment was prepared in a duplicate and stored at either 22°C or 1°C. Viability was measured after 31 days. Data shown in the chart to the right. No other statistics were performed (there were no statistically significant differences between the different types of storage mediums at room temperature). All storage mediums shared results similar to Richard's results <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1115768398451373/?comment_id=1145139948847551&offset=0&total_comments=69&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Conversation with Mark Trent on MTF. 09/10/2015.]</ref>. It appears from these experiments that ''Brettanomyces'' stores better cold when stored for less than a month, however these results need to be verified and more strains should be examined before broad statements can be applied about the effects of temperature when storing ''Brettanomyces'' in wort or MYPG. Interestingly, the current study suggests that '''after''' one month, ''Brettanomyces'' survival rate is generally stronger at room temperatures than at refrigeration temperatures.