Difference between revisions of "Laboratory Techniques"

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(→‎Brettanomyces)
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====''Brettanomyces''====
 
====''Brettanomyces''====
  
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A few different medias can be used to isolate ''Brettanomyces'' but DBDM media is commonly used. WLN and WLD with additions of cycloheximide can also be used.  
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A few different medias can be used to isolate ''Brettanomyces'' but DBDM media is commonly used. WLD with additions of cycloheximide can also be used.  
  
 
'''DBDM Media Recipe'''
 
'''DBDM Media Recipe'''
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|-
 
|-
 
| p-coumaric acid || 100 mg
 
| p-coumaric acid || 100 mg
 +
|-
 +
| Bromocresol Green || 22 mg
 +
|-
 +
| Agar || 20 grams
 +
|-
 +
| Distilled Water || Fill to 1000 ML
 +
|}
 +
 +
'''WLD Media Recipe'''
 +
{| class="wikitable"
 +
|-
 +
! Chemical !! Usage Amount
 +
|-
 +
| Cycloheximide || 4 grams
 +
|-
 +
| Yeast Extract || 4 grams
 +
|-
 +
| Pancreatic Digest of Casein(Pepton) || 5 grams
 +
|-
 +
| Dextrose || 50
 +
|-
 +
| Monopotassium Phosphate || .55 grams
 +
|-
 +
| Potassium Chloride || 425 mg
 +
|-
 +
| Calcium Chloride || 125 mg
 +
|-
 +
| Magnesium Sulfate || 125 mg
 +
|-
 +
| Ferric Chloride || 2.5 mg
 +
|-
 +
| Manganese Sulfate || 2.5 mg
 
|-
 
|-
 
| Bromocresol Green || 22 mg
 
| Bromocresol Green || 22 mg

Revision as of 06:29, 1 September 2017

This page will focus on home lab and small brewery lab techniques.

Equipment

(To do)

Growth Media

Lactobacillus

See Rogosa SL Agar

Saccharomyces

Brettanomyces

A few different medias can be used to isolate Brettanomyces but DBDM media is commonly used. WLD with additions of cycloheximide can also be used.

DBDM Media Recipe

Chemical Usage Amount
Yeast nitrogenous base (YNB) 6.5 grams
Ethanol 6% v/v
Cycloheximide 10 mg
p-coumaric acid 100 mg
Bromocresol Green 22 mg
Agar 20 grams
Distilled Water Fill to 1000 ML

WLD Media Recipe

Chemical Usage Amount
Cycloheximide 4 grams
Yeast Extract 4 grams
Pancreatic Digest of Casein(Pepton) 5 grams
Dextrose 50
Monopotassium Phosphate .55 grams
Potassium Chloride 425 mg
Calcium Chloride 125 mg
Magnesium Sulfate 125 mg
Ferric Chloride 2.5 mg
Manganese Sulfate 2.5 mg
Bromocresol Green 22 mg
Agar 20 grams
Distilled Water Fill to 1000 ML

Reference on DBDM: https://www.facebook.com/groups/MilkTheFunk/permalink/1805210829507123/?comment_id=1805397166155156&comment_tracking=%7B%22tn%22%3A%22R%22%7D

Misc/Other

Propagators

Techniques

(Videos provided by Bryan of Sui Generis blog and Zach Taggart.)

Aseptic Technique

Making Agar Plates

  • Making WLD plates:

Yeast/Bacteria Isolation

Making Your Own Medias

Yeast Banking

Cell Counting

Gram Staining

Shipping Cultures

Example of DeWayne Schaaf's packaging for shipping cultures.

DeWayne Schaaf recommends the following procedures for shipping cultures with the USA states and territories [1]:

  1. Use 15ml centrifuge tubes from Cynmar LLC - Wine & Brew. They are great quality and have a very low rate of leakage, especially when combined with electrical tape.
  1. Place each liquid filled tube into its own snack sized baggie to minimize cross contamination if they do happen to leak.
  1. Each of these smaller bags will be placed into a quart Ziplock.
  1. My main shipping container are Uline Poly bubble mailers. I don't tend to use ice packs as I've found them unnecessary when shipping in favorable temps (use ice packs during the summer).
  1. USPS is my preferred shipping method. Using first class shipping, my packages typically take no more than 3 days to reach somewhere in the USA and cost right around $3, and that includes a tracking number. Shipping rates are roughly the same for any USA state or territory. The 48 continental states, Alaska, Hawaii, Puerto Rico, and Guam all cost roughly the same.

See also:

See Also

Additional Articles on MTF Wiki

External Resources

References

  1. ↑ DeWayne Schaaf. Kveik World Order Facebook page. 04/21/2017. Retrieved 04/24/2017.
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