Difference between revisions of "Wild Yeast Isolation"
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| − | + | '''Wild yeast isolation''', sometimes known as ''yeast wrangling'', refers to the process of catching wild yeast, and isolating it with agar plates as a pure culture. This article will also contain information for growing up a captured mixed culture in wort rather than isolating pure cultures. | |
| − | + | :''Special thanks goes out to Bryan Heit from Sui Generis blog and Jeff Mello of Bootleg Biology for providing the information in this article.'' | |
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| − | ==Isolating== | + | ==Isolating Pure Cultures== |
| − | === | + | ===Preparation=== |
| − | ===Without | + | * [http://suigenerisbrewing.blogspot.ca/p/yeast-wrangling.html Bryan Heit's] video guide to setting up a clean workspace, building and using an alcohol lamp, and aseptic techniques. |
| + | * [http://bootlegbiology.com/diy/creating-plates/ Bootleg Biology's] guide on creating agar plates using wort, agar powder, and yeast nutrient. | ||
| + | * [http://suigenerisbrewing.blogspot.ca/2015/03/new-video-casting-agar-plates.html Bryan Heit's] guide to more types of agar plates, and video tutorial. | ||
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| + | ===Catching=== | ||
| + | * [http://bootlegbiology.com/diy/capturing-yeast/ Bootleg Biology] defines three methods of catching a wild yeast. | ||
| + | * [http://suigenerisbrewing.blogspot.ca/2014/12/two-new-videos.html Bryan Heit's] video tutorial for catching wild yeast from fruit or from the air. | ||
| + | |||
| + | ===Isolating=== | ||
| + | * [http://bootlegbiology.com/diy/isolating-yeast/ Bootleg Biology's] guide to streaking agar plates to isolate the captured yeast cultures. | ||
| + | * [http://suigenerisbrewing.blogspot.ca/2014/08/your-home-yeast-lab-made-easy-streak.html Bryan Heit's] video guide on how to streak agar plates. | ||
| + | * [http://suigenerisbrewing.blogspot.ca/2014/12/two-new-videos.html Bryan Heit's] video guide on streaking plates and further isolating yeast. | ||
| + | |||
| + | ==Growing Without Isolation== | ||
| + | (To do) | ||
==See Also== | ==See Also== | ||
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==External Resources== | ==External Resources== | ||
| − | * [http://bootlegbiology. | + | * [http://bootlegbiology.comBootleg Biology provides a full toolkit and materials for yeast isolation.] |
| − | * [ | + | * [http://suigenerisbrewing.blogspot.ca ''Sui Generis'' blog by Bryan Heit.] |
* [https://bkyeast.wordpress.com/2012/12/17/how-to-build-a-yeast-ranch/ BKYeast, ''How to Build a Yeast Ranch''.] | * [https://bkyeast.wordpress.com/2012/12/17/how-to-build-a-yeast-ranch/ BKYeast, ''How to Build a Yeast Ranch''.] | ||
==References== | ==References== | ||
<references/> | <references/> | ||
Revision as of 22:48, 20 May 2015
Wild yeast isolation, sometimes known as yeast wrangling, refers to the process of catching wild yeast, and isolating it with agar plates as a pure culture. This article will also contain information for growing up a captured mixed culture in wort rather than isolating pure cultures.
- Special thanks goes out to Bryan Heit from Sui Generis blog and Jeff Mello of Bootleg Biology for providing the information in this article.
Contents
Isolating Pure Cultures
Preparation
- Bryan Heit's video guide to setting up a clean workspace, building and using an alcohol lamp, and aseptic techniques.
- Bootleg Biology's guide on creating agar plates using wort, agar powder, and yeast nutrient.
- Bryan Heit's guide to more types of agar plates, and video tutorial.
Catching
- Bootleg Biology defines three methods of catching a wild yeast.
- Bryan Heit's video tutorial for catching wild yeast from fruit or from the air.
Isolating
- Bootleg Biology's guide to streaking agar plates to isolate the captured yeast cultures.
- Bryan Heit's video guide on how to streak agar plates.
- Bryan Heit's video guide on streaking plates and further isolating yeast.
Growing Without Isolation
(To do)
See Also
External Resources
- Biology provides a full toolkit and materials for yeast isolation.
- Sui Generis blog by Bryan Heit.
- BKYeast, How to Build a Yeast Ranch.