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'''Packaging''' is the transfer of your mixed fermentation/sour/funky beer from a fermentation or aging vessel into the the final package (e.g. a bottle or a keg). This page will discuss various things one should consider such as when to package, how to package and what final package to put the beer into. See [[Aging and Storage]] for storage information.
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'''Packaging''' is the transfer of your mixed fermentation/sour/funky beer from a fermentation or aging vessel into the the final package (e.g. a bottle or a keg). This page will discuss various things one should consider such as when to package, how to package and what final package to put the beer into. For information on packaging with corks and in large format bottles, see the [[Corking]] page. See [[Aging and Storage]] for storage information.
  
 
==When to package==
 
==When to package==
(in progress)
 
 
===Final Gravity===  
 
===Final Gravity===  
  
There is no hard and fast rule for when to package based on final gravity because the gravity of the finished beer will depend on recipe, process and microbes present among other factors. Generally lower final gravities are safer because they constrain the extent to which the beer can continue to ferment and generate extra CO2 in the package. But rather than looking for a specific number, pay more attention to the stability of the gravity. Jay from the Rare Barrel recommends waiting at the final gravity for 2-3 months in mixed fermentation beers to ensure that this gravity is the actual final gravity <ref name='Sour Hour episode 6'>[http://www.thebrewingnetwork.com/the-sour-hour-episode-6/ The Sour Hour episode 6 Listener Q&A]</ref> (~38 minutes in). If the gravity is stable over a long time scale (weeks to months, depending again on factors such as recipe, process and microbes present), then the beers is probably done. If you are unsure then give the beer a bit longer and monitor final gravity. Each gravity point (.001) of continued fermentation yields roughly 1/2 volume of CO2.
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There is no hard and fast rule for when to package based on final gravity because the gravity of the finished beer will depend on recipe, process, and microbes present among other factors. Generally, lower final gravities are safer because they constrain the extent to which the beer can continue to ferment and generate extra CO<sup>2</sup> in the package. But rather than looking for a specific number, pay more attention to the stability of the gravity. Jay from the Rare Barrel recommends waiting at the final gravity for 2-3 months in mixed fermentation beers to ensure that this gravity is the actual final gravity <ref name='Sour Hour episode 6'>[http://www.thebrewingnetwork.com/the-sour-hour-episode-6/ The Sour Hour episode 6 Listener Q&A]</ref> (~38 minutes in). If the gravity is stable over a long time scale (weeks to months, depending again on factors such as the recipe, process, and microbes present), then the beer is probably done. If you are unsure then give the beer a bit longer and monitor final gravity. Keep in mind that sampling sour beer too often can lead to too much oxygen exposure, and care should be taken not not sample too much and purge with CO2; see [[Mixed_Fermentation#Aging|Mixed Fermentation]] for more information. Each gravity point (.001) of continued fermentation yields roughly 1/2 volume of CO2 (each degree Plato yields 2 volumes of CO2) <ref>[http://braukaiser.com/wiki/index.php/Accurately_Calculating_Sugar_Additions_for_Carbonation#Remaining_or_Residual_Extract "Accurately Calculating Sugar Additions for Carbonation."  Kai Troester.  Braukaiser.com.  Retrieved 08/07/2016.]</ref>.  Sometimes beer samples will contain high amounts of carbonation due to gradual fermentation from ''Brettanomyces'' (in other cases when the beer has aged for a very long time, there may be much less residual carbonation than normal).  Therefore, it is best practice to degas the sample by pouring it through a membrane filter such as a coffee filter in order to obtain an accurate gravity reading <ref>[http://methods.asbcnet.org/summaries/degassingmatrix.aspx "Recommended Beer Degassing Methods and Alternatives Matrix".  ASBC Methods of Analysis.  Retrieved 03/04/2020.]</ref>. 
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With continual batches of beer using the same grist and strains of microbes, a stable final gravity can become more easily predicted by the brewer and waiting for a stable final gravity may no longer be necessary once a known stable final gravity is achieved (note that a change in the strains being used or the grist could result in a different stable final gravity).  For example, see the [[Brettanomyces and Saccharomyces Co-fermentation#Dosing_Clean_Beer_with_Brettanomyces_At_Bottling|Brettanomyces and Saccharomyces Co-fermentation]] page for techniques for inoculating ''Brettanomyces'' at bottling time into a clean beer that was fermented with just ''S. cerevisiae''. 
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If fruit was added to an already matured mixed fermentation beer with living ''Brettanomyces'' in it, an additional 1-2 months should be given after adding the fruit to ensure that the sugars from the fruit are fully fermented.  See [[Soured_Fruit_Beer#Refermentation|Soured Fruit Beer Refermentation]] for details on using fruit in sour beers.
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For suggested equipment for reading gravity from small samples, see [[Packaging#Density_Meters|Density Meters]].
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===Flavor Development===
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While determining if the final gravity is stable is the most important factor when choosing when to package mixed fermentation beer, there are also different approaches to choosing when to package beer based on the flavor maturity of the beer.  For [[Mixed Fermentation]] beers that contain ''Brettanomyces'' and lactic acid bacteria, months are often required for the beer to reach an optimal or even just acceptable flavor profile.  This depends on many variables such as is the beer aging in an oak [[Barrel]], what microbes are pitched (for example, ''Pediococcus'' and slower species of ''Lactobacillus'' such as ''L. delbruekii'' tend to need a few months to develop acidity), what flavors the brewer is looking for, etc.  If the gravity is stable, some brewers prefer the option of packaging the beer before the flavor is optimal and allowing the flavor to continue to develop in the bottle/keg.  This gives the advantage of being able to sample the beer at different points in time and can serve as a good technique for learning how a particular culture ages.  If this approach is taken, keeping the bottles/kegs at room temperature instead of at refrigerator or cellar temperatures is advised so that the yeast and bacteria do not fall dormant.  Colder temperatures will slow the maturation of the beer.  Other brewers prefer to allow the maturation of flavors to happen in the fermenting vessel (especially in the case of oak barrels), and then package the beer when it tastes good.  This approach is less risky as far as potentially packaging a premature beer that never fully reaches the flavor profile that the brewer finds acceptable.  In other words, sometimes mixed fermentation beers never taste good, and allowing the beer to bulk age in the fermenting vessel allows the brewer to make sure the beer is worth packaging. 
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For more information on when mixed fermentation beers are ready to package based on flavor, see the various [[Table_of_Contents#Brewing_Techniques|Brewing Techniques]] pages. For more information on aging and cellaring packaged beer, see [[Aging and Storage]].
  
 
===Clarity and pellicles===  
 
===Clarity and pellicles===  
Beer with ''Brettanomyces'' and bacteria, as well as certain ''Saccharomyces'' strains, will take longer to clear than other beers. Generally time can take care of most of the clarification in mixed fermentation beers with long aging times. Faster turnaround beers or kettle sours may not clarify by time alone. The level of clarity you are looking for before packaging will depend on your preferences and the beer you are making. Beer can be packaged without good clarity and they will continue to settle out in the bottle, but note that beers with high carbonation levels may kick the sediment back into suspension, which may or may not be what you want in your beer.
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Beer with ''Brettanomyces'' and bacteria, as well as certain ''Saccharomyces'' strains, will take longer to clear than other beers. Generally, time can take care of most of the clarification in mixed fermentation beers with long aging times. Faster turnaround beers or kettle sours may not clarify by time alone. The level of clarity you are looking for before packaging will depend on your preferences and the beer you are making. Beer can be packaged without good clarity and they will continue to settle out in the bottle, but note that beers with high carbonation levels may kick the sediment back into suspension, which may or may not be what you want in your beer.
  
 
Some producers will coarse filter their fruited or dry hopped sour beers to prevent pieces of fruit from clogging equipment or making it to the package <ref>[https://www.facebook.com/CaseyBrewingAndBlending/photos/a.650486161661121.1073741829.625431407499930/900252466684488/?type=3 Casey Brewing & Blending fruit strainer]</ref><ref>[https://www.youtube.com/watch?v=vDCoSJzP_18 Jean at Cantillon making a filter]</ref>
 
Some producers will coarse filter their fruited or dry hopped sour beers to prevent pieces of fruit from clogging equipment or making it to the package <ref>[https://www.facebook.com/CaseyBrewingAndBlending/photos/a.650486161661121.1073741829.625431407499930/900252466684488/?type=3 Casey Brewing & Blending fruit strainer]</ref><ref>[https://www.youtube.com/watch?v=vDCoSJzP_18 Jean at Cantillon making a filter]</ref>
  
Beer with a pellicle can be bottled and it is not necessary to wait for a pellicle to disappear before bottling.
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Beer with a pellicle can be bottled, and it is not necessary to wait for a pellicle to disappear before bottling.  See [[Pellicle#Introduction|Pellicle]] for more information.
  
 
==How to package==
 
==How to package==
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===General===
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* [https://www.facebook.com/groups/MilkTheFunk/permalink/4448138071881039 General MTF thread for commercial brewers on packaging mixed fermentation beer.]
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* [https://www.facebook.com/groups/MilkTheFunk/permalink/1244463818915163/ MTF Thread on 4-6 spout gravity-fed bottling lines.]
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* [https://www.facebook.com/groups/MilkTheFunk/permalink/1368944336467110/ MTF Thread for advice on how commercial brewers use blending/bottling tanks.]
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* [https://www.facebook.com/groups/MilkTheFunk/permalink/1324183294276548/ MTF discussion on commercial bottle fillers.]
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===Re-yeasting===
 
===Re-yeasting===
 
When bottling or priming a keg at packaging time, the brewer should consider re-yeasting.  In a long aged beer, especially an acidic beer, the ''Saccharomyces'' will mostly be dead already.  This leaves ''Brettanomyces'' in charge of conditioning the beer.  Although some lactic acid bacteria are capable of producing CO2, their contribution is probably negligible.  It is also possible that homofermentative LAB will consume a portion of the sugar before the ''Brett'' has a chance to produce CO2.  Re-yeasting is a very effective way to ensure proper CO2 levels in an aged sour beer.  Another benefit of re-yeasting is that it tends to help avoid (or minimize) [[Tetrahydropyridine]] production.
 
When bottling or priming a keg at packaging time, the brewer should consider re-yeasting.  In a long aged beer, especially an acidic beer, the ''Saccharomyces'' will mostly be dead already.  This leaves ''Brettanomyces'' in charge of conditioning the beer.  Although some lactic acid bacteria are capable of producing CO2, their contribution is probably negligible.  It is also possible that homofermentative LAB will consume a portion of the sugar before the ''Brett'' has a chance to produce CO2.  Re-yeasting is a very effective way to ensure proper CO2 levels in an aged sour beer.  Another benefit of re-yeasting is that it tends to help avoid (or minimize) [[Tetrahydropyridine]] production.
  
Commercial producers and MTFers have had success re-yeasting with their mixed culture, wine yeast, and champagne yeast.  The specific yeast you choose is up to you, and we recommend that you try a couple different yeasts out to find the one you prefer. When re-yeasting at bottling, it is not recommended to add new highly attenuative yeast to make sure that the bottling yeast you add cannot ferment additional carbohydrates remaining in the beer <ref name='Sour Hour episode 6'/> (~41 minutes in). Re-yeasting with a yeast that beer has already seen should eliminate the possibility of continued attenuation as long as the beer is already at final gravity.  Brewers who are having difficulty carbonating sour beer or need to ensure that the beer will carbonate properly should grow their yeast first in a yeast starter that has yeast nutrients (Fermaid K + DAP, for example) and diluted 1:1 with the sour beer itself.  This will acclimate the yeast to the harsh conditions of the sour beer, and has shown to be more effective for ensuring bottle carbonation <ref>[http://www.sciencedirect.com/science/article/pii/S0740002016301605 Terminal acidic shock inhibits sour beer bottle conditioning by Saccharomyces cerevisiae.  Cody M. Rogers, Devon Veatch, Adam Covey, Caleb Staton, Matthew L. Bochman.  2016.]</ref>.  See [[Saccharomyces#Fermentation_Under_Low_pH_Conditions|Fermenting in low pH conditions]] for more information.
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Commercial producers and MTFers have had success re-yeasting with their mixed culture, wine yeast, and champagne yeast.  The specific yeast you choose is up to you, and we recommend that you try a couple different yeasts out to find the one you prefer (MTFers have reported success with Lalvin EC-1118 Champagne, Red Star Premier Cuvée, Red Star Pasteur Blanc, Danstar CBC-1, Fermentis T-58, Scott Laboratories DV10 wine yeast, and fresh cultures of ''Brettanomyces'' <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1477763322251877/ Conversation on MTF regarding bottle conditioning yeast.  11/21/2016.]</ref>). When re-yeasting at bottling for a beer that has some residual gravity (a higher than 0 plato or specific gravity), take care not to use a highly attenuative yeast that could over-attenuate the beer in the package.  For example, adding ''Brettanomyces'' or a diastatic strain of ''Saccharomyces cerevisiae'' at bottling to a beer that has some residual sugars could result in over-carbonation in the bottle <ref name='Sour Hour episode 6'/> (~41 minutes in). If the beer has a significantly high finishing gravity, then re-yeasting with a yeast that beer has already seen should eliminate the possibility of continued attenuation as long as the beer is already at final gravity.  Using rehydrated dry yeast has been shown to be more reliable than both slurries and even freshly propagated yeast probably due to their rich sterol reserves (this excludes freshly propagated yeast using acid shock starters - see below) <ref>[https://www.researchgate.net/publication/293337388_Effect_of_pitching_yeast_preparation_on_the_refermentation_of_beer_in_bottles an Landschoot, A., Vanbeneden, N., Vanderputten, D. and Derdelinckx, G. (2004). Effect of pitching yeast preparation on the refermentation of beer in bottles. Cerevisia 29:140-147.]</ref><ref>Van den Berg, S., Demeyere, K. and Van Landschoot, A. (2001). The use of dried yeast for the bottle refermentation of beer. Cerevisia 26:102-108.</ref>.  Brewers who are having difficulty carbonating sour beer or need to ensure that the beer will carbonate properly should grow their yeast first in an [[Packaging#Acid_Shock_Starters|Acid Shock Starter]].  This will acclimate the yeast to the harsh conditions of the sour beer, and has shown to be more effective for ensuring bottle carbonation <ref>[http://www.sciencedirect.com/science/article/pii/S0740002016301605 Terminal acidic shock inhibits sour beer bottle conditioning by Saccharomyces cerevisiae.  Cody M. Rogers, Devon Veatch, Adam Covey, Caleb Staton, Matthew L. Bochman.  2016.]</ref>.  Not all sour beers will need the priming yeast to be acclimated, but sour beers with darker malts, acidic fruits, and high ABV (8%+) might require this step.  See [[Packaging#Acid_Shock_Starters|Acid Shock Starters]] below, and [[Saccharomyces#Fermentation_Under_Low_pH_Conditions|fermenting in low pH conditions]] for more information on the science behind this technique.
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The yeast required for carbonation is very little.  A good rule of thumb to use is to use 10% of the yeast that you would normally use for a primary fermentation (approximately 1 million cells per mL).  For example, for dried yeast use ~2 grams of yeast for 5 gallons of beer <ref name="priming_calc">[http://jeffreycrane.blogspot.com/2015/06/blending-calculator-ph-abv-and.html Blending Calculator - pH, ABV and Carbonation.  Jeff Crane.  Blending Calculator - pH, ABV and Carbonation.  Bikes, Beer, & Adventures Blog.  June 12, 2015.]</ref>.  Rehydrating the yeast is recommended.  See [http://jeffreycrane.blogspot.com/2015/06/blending-calculator-ph-abv-and.html Jeff Crane's "Blending Calculator" (extension of Michael Tonsmeire's "Blending Calculator")] for a re-yeasting and priming calculator.  Dry yeast should be rehydrated in ten times its weight in 80-90°F (27-32°C) water for ~15-20 minutes <ref name="Zandycke_Bottling">[http://www.mbaa.com/publications/tq/tqPastIssues/2011/Abstracts/TQ-48-1-0225-01.htm S. M. Van Zandycke, T. Fischborn, D. Peterson, G. Oliver, and C. D. Powell (2011).  The Use of Dry Yeast for Bottle Conditioning.  MBAA Technical Quarterly.  doi:10.1094/TQ-48-1-0225-01.]</ref>.
  
The yeast required for carbonation is very littleA good rule of thumb to use is to use 10% of the yeast that you would normally use for a primary fermentation (approximately 1 million cells per mL)For example, for dried yeast use ~2 grams of yeast for 5 gallons of beer <ref name="priming_calc">[http://jeffreycrane.blogspot.com/2015/06/blending-calculator-ph-abv-and.html Blending Calculator - pH, ABV and Carbonation.  Jeff CraneBlending Calculator - pH, ABV and Carbonation.  Bikes, Beer, & Adventures BlogJune 12, 2015.]</ref>.  Rehydrating the yeast is recommended.  See [http://jeffreycrane.blogspot.com/2015/06/blending-calculator-ph-abv-and.html Jeff Crane's "Blending Calculator" (extension of Michael Tonsmeire's "Blending Calculator")] for a re-yeasting and priming calculator.
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Many wine yeast strains are known to be "killer" yeast strains.  In ''Saccharomyces'', killer strains produce toxins that kill sensitive strainsNeutral strains do not produce toxins, nor are they killed by them <ref>[https://books.google.com/books?hl=en&lr=&id=mvORN6OXHh4C&oi=fnd&pg=PA93&dq=Bussey,+H.+1981.+Physiology+of+killer+factor+in+yeast.+Adv.+Microb.+Physiol.+22:93-121&ots=jUY4T9NpgB&sig=aw-Y1um0KsDnGe6rRe5PTWIDYdI#v=onepage&q&f=false Advances in Microbial Physiology, Volume 22.  Academic Press, Sep 15, 1981.  Pg 94-95.]</ref>.  Almost all ale and lager strains are sensitive to the toxins produced by killer strains <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1179271825434363/?comment_id=1179424538752425&offset=0&total_comments=5&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with Bryan of Sui Generis Blog on MTF on Killer Factor for Saccharomyces.  11/16/2015.]</ref><ref>[http://onlinelibrary.wiley.com/doi/10.1002/j.2050-0416.1973.tb03515.x/pdf Strains of Yeast Lethal to Brewery Yeasts.  A.P. Maule and P.D. Thomas.  1972.]</ref>In ''Saccharomyces'', four toxins have been identified: K1, K2, K28, and Klus, the first three of which can only kill other strains/species of ''Saccharomyces''.  The Klus toxin has been found to kill yeast from other genra, such as ''Hanseniaspora spp.'', ''Kluyveromyces lactis'', ''Candida albicans'', ''Candida dubliniensis'', ''Candida kefir'' and ''Candida tropicalis'', and the K1, K2 and K28 killer strains of ''S. cerevisiae'' <ref name="Rodriguez">[http://aem.asm.org/content/77/5/1822.long A New Wine Saccharomyces cerevisiae Killer Toxin (Klus), Encoded by a Double-Stranded RNA Virus, with Broad Antifungal Activity Is Evolutionarily Related to a Chromosomal Host GeneNieves Rodríguez-Cousiño, Matilde Maqueda, Jesús Ambrona, Emiliano Zamora, Rosa Esteban and Manuel Ramírez2011]</ref>.  However, none of the toxins have been found to kill ''Brettanomyces'' <ref>[http://www.scielo.org.za/scielo.php?pid=S2224-79042015000100010&script=sci_arttext&tlng=pt Non-Saccharomyces killer toxins: Possible biocontrol agents against Brettanomyces in wine?  S. Afr. J. Enol. Vitic. vol.36 n.1 Stellenbosch.  2015.]</ref>.  The K1 toxin is most active between a pH of 4.6 and 4.8, while K2 and Klus are active around a pH of 4.0 to 4.3 <ref name="Rodriguez"></ref>.  See [[Saccharomyces#Killer_Wine_Yeast|Killer Wine Yeast]] for more information on these toxins.  
  
Many wine yeast strains are known to be "killer" yeast strains.  In ''Saccharomyces'', killer strains produce toxins that kill sensitive strains.  Neutral strains do not produce toxins, nor are they killed by them <ref>[https://books.google.com/books?hl=en&lr=&id=mvORN6OXHh4C&oi=fnd&pg=PA93&dq=Bussey,+H.+1981.+Physiology+of+killer+factor+in+yeast.+Adv.+Microb.+Physiol.+22:93-121&ots=jUY4T9NpgB&sig=aw-Y1um0KsDnGe6rRe5PTWIDYdI#v=onepage&q&f=false Advances in Microbial Physiology, Volume 22.  Academic Press, Sep 15, 1981.  Pg 94-95.]</ref>.  Almost all ale and lager strains are sensitive to the toxins produced by killer strains <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1179271825434363/?comment_id=1179424538752425&offset=0&total_comments=5&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with Bryan of Sui Generis Blog on MTF on Killer Factor for Saccharomyces11/16/2015.]</ref><ref>[http://onlinelibrary.wiley.com/doi/10.1002/j.2050-0416.1973.tb03515.x/pdf Strains of Yeast Lethal to Brewery YeastsA.P. Maule and P.D. Thomas.  1972.]</ref>.  In ''Saccharomyces'', four toxins have been identified: K1, K2, K28, and Klus, the first three of which can only kill other strains/species of ''Saccharomyces''.  The Klus toxin has been found to kill yeast from other genra, such as ''Hanseniaspora spp.'', ''Kluyveromyces lactis'', ''Candida albicans'', ''Candida dubliniensis'', ''Candida kefir'' and ''Candida tropicalis'', and the K1, K2 and K28 killer strains of ''S. cerevisiae'' <ref name="Rodriguez">[http://aem.asm.org/content/77/5/1822.long A New Wine Saccharomyces cerevisiae Killer Toxin (Klus), Encoded by a Double-Stranded RNA Virus, with Broad Antifungal Activity Is Evolutionarily Related to a Chromosomal Host Gene.  Nieves Rodríguez-Cousiño, Matilde Maqueda, Jesús Ambrona, Emiliano Zamora, Rosa Esteban and Manuel Ramírez.  2011]</ref>However, none of the toxins have been found to kill ''Brettanomyces'' <ref>[http://www.scielo.org.za/scielo.php?pid=S2224-79042015000100010&script=sci_arttext&tlng=pt Non-Saccharomyces killer toxins: Possible biocontrol agents against Brettanomyces in wine?  S. Afr. J. Enol. Vitic. vol.36 n.1 Stellenbosch.  2015.]</ref>.  The K1 toxin is most active between a pH of 4.6 and 4.8, while K2 and Klus are active around a pH of 4.0 to 4.3 <ref name="Rodriguez"></ref>.
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This tends to create some fears for brewers who believe that the ''Saccharomyces'' strains in their beer might still be aliveHowever, in a highly acidic sour beer, ''Saccharomyces'' wine strains tend not to live for extended periods of time <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1268830686478476/?comment_id=1269605776400967&reply_comment_id=1270016239693254&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with Richard Preiss on MTF.  03/28/2016.]</ref>. Even if some cells do, their activity would be next to none due to no available sugars left in the beer (other than priming sugar), and thus their contribution to the flavor development of the beer would be minimal to noneAssuming good bottling practices are followed, fear of autolysis occurring due to killer strain activity is usually unfounded because most of the ''Saccharomyces'' cells are left behind with the trub at bottlingAdditionally, ''Brettanomyces'' has the ability to metabolize the acids and proteins that are released during whatever autolysis that might occur in the bottle. Re-yeasting with wine yeast for priming has the additional advantage of not fermenting maltose or maltotriose, so unexpected attenuation from the wine yeast will generally not occur as long as the beer is already fully attenuatedThus, there is little argument against re-yeasting with wine yeast at packaging time, other than a desire to approach carbonation in a traditional sense such as is the case with [[lambic]].
  
This tends to create some fears for brewers who believe that the ''Saccharomyces'' strains in their beer might still be alive.  However, in a highly acidic sour beer, ''Saccharomyces'' wine strains tend not to live for extended periods of time <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1268830686478476/?comment_id=1269605776400967&reply_comment_id=1270016239693254&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with Richard Preiss on MTF.  03/28/2016.]</ref>.  Even if some cells do, their activity would be next to none due to no available sugars left in the beer (other than priming sugar), and thus their contribution to the flavor development of the beer would be minimal to noneAssuming good bottling practices are followed, fear of autolysis occurring due to killer strain activity is usually unfounded because most of the ''Saccharomyces'' cells are left behind with the trub at bottlingAdditionally, ''Brettanomyces'' has the ability to metabolize the acids and proteins that are released during whatever autolysis that might occur in the bottleRe-yeasting with wine yeast for priming has the additional advantage of not fermenting maltose or maltotriose, so unexpected attenuation from the wine yeast will generally not occur as long as the beer is already fully attenuatedFlavor impact by the wine yeast is also probably not significant due to the small amount of fermentables provided by the priming sugar. Thus, there is little argument against re-yeasting with wine yeast at packaging time, other than a desire to approach carbonation in a traditional sense such as is the case with [[lambic]].
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The flavor impact by the wine yeast in beers that contain living ''Brettanomyces'' is also probably minimal or not significant, although this has yet to be established scientifically <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1584074608287414/?comment_id=1584083038286571&reply_comment_id=1584163181611890&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with Richard Preiss on MTF.  02/15/2017.]</ref>.  This would be due to the small amount of fermentables provided by the priming sugar, as well as the ability of ''Brettanomyces'' to metabolize many secondary metabolites that would be produced by a bottling yeastIt has been shown, however, that bottling yeast in general can have a flavor impact on clean beers without ''Brettanomyces''.  For example one study found that out of 5 dried yeast strains from the Lallemand Yeast Culture Collection (Montreal, QC, Canada), one strain produced a higher final pH by about ~.05 (from 3.98 to 4.02 pH) in a Belgian dark ale (no ''Brettanomyces'' or other non-traditional microbes were used in the beer)The various strains tested also affected the levels of acetic acid, ethyl acetate, isoamyl alcohol, acetaldehyde, diacetyl, and glycerol in beer just from bottle conditioning <ref name="Zandycke_Bottling" />Some brewers speculate that thiols from wine yeast could also create novel characteristics in beer (although perhaps not from only bottle conditioning yeast) <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1380430375318506/ Conversation with James Sites about thiol producing wine strains on MTF.  08/19/2016.]</ref>. It could therefore be possible that metabolites produced from bottling yeast could have an effect on sour/"Brett" beer flavor as well, but this remains to be demonstrated scientifically.
  
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* See [[Saccharomyces#Killer_Wine_Yeast|Killer Wine Yeast]] for more information.
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* See [[Funky_Mixed_Fermentations#Dosing_Clean_Beer_with_Brettanomyces_At_Bottling|Bottling Clean Beer with Brettanomyces]] for information on dosing bottles of beer with ''Brettanomyces'' at bottling time.
 
* [http://www.math.fsu.edu/~gmizell/mead/Lallemand%20Yeast%20Quick%20Reference.pdf Lallemand chart of wine yeasts and their Killer Factor.]
 
* [http://www.math.fsu.edu/~gmizell/mead/Lallemand%20Yeast%20Quick%20Reference.pdf Lallemand chart of wine yeasts and their Killer Factor.]
 
* [http://www.lewybrewing.com/2012/12/wine-yeast-in-beer-experiment.html?m=1 Chris Lewis's write up on wine yeast character/Killer Factor on Lewy Brewing Blog.]
 
* [http://www.lewybrewing.com/2012/12/wine-yeast-in-beer-experiment.html?m=1 Chris Lewis's write up on wine yeast character/Killer Factor on Lewy Brewing Blog.]
 
* [http://www.babblebelt.com/newboard/thread.html?tid=1108752780&th=1275037001 Shea Comfort notes on wine yeast on the BBB.]
 
* [http://www.babblebelt.com/newboard/thread.html?tid=1108752780&th=1275037001 Shea Comfort notes on wine yeast on the BBB.]
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====Acid Shock Starters====
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Below are several ''acid shock starter'' techniques for acclimating yeast to the harsh conditions of acidic wort or beer, including mixed fermentation sour beers and kettle sour beers.  See [[Saccharomyces#Fermentation_Under_Low_pH_Conditions|fermenting ''Saccharomyces'' under low pH conditions]] for more information on the science behind this. 
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* See also [https://web.archive.org/web/20211023133240/https://www.therarebarrel.com/index.cfm?method=blog.blogDrilldown&blogEntryID=F8F5CB68-B1C7-9CB1-ACBD-546329AE621D&originalMarketingURL=blog/Terminal-Acidic-Shock-and-Sour-Ale-Bottle-Conditioning The Rare Barrel's acid shock treatment].
 +
* See also [https://www.facebook.com/groups/MilkTheFunk/permalink/4429403613754485 this MTF thread] for updates on acclimation methods and reported experiences.
 +
 +
<blockquote style="background-color: lightgrey; border: solid thin grey; padding:10px;">
 +
'''Escarpment Labs Acclimation Protocol'''
 +
 +
The following acclimation protocol has been adapted for sour beer from Richard Preiss's instructions on how Escarpment Labs acclimates wine yeast: <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1363395580355319/?comment_id=1364119323616278&reply_comment_id=1364521000242777&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Wine yeast acclimation protocol for Escarpment Labs by Richard Preiss.  08/01/2016.]</ref>
 +
 +
# Take a "pinch" of rehydrated dried yeast, and pitch 150,000 cells/mL into 100 mL of '''YPD + Go-Ferm Media'''. Grow for ~24 hours at 86°F (30°C) with shaking.
 +
# Transfer the starter into 100 mL of '''Adaption Media 1'''.  Grow for ~24 hours at 86°F (30°C) without shaking.
 +
# Transfer the starter into '''Adaption Media 2''' (whatever volume is needed for batch size).  Grow for ~24 hours at 86°F (30°C) without shaking.
 +
# Pitch ~1 million cells per mL of sour beer that is being carbonated, along with priming sugar.
 +
 +
Definitions:
 +
 +
;YPD + Go-Ferm Media (autoclaved):
 +
: 0.02375 g/L Go-Ferm
 +
: 10 g/L yeast extract
 +
: 20 g/L peptone
 +
: 20 g/L dextrose
 +
 +
;Adaption Media 1 (filter-sterilized)
 +
: 10% base sour beer
 +
: 20 g/L sucrose
 +
: 0.02375 g/L Go-Ferm
 +
 +
;Adaption Media 2 (filter-sterilized)
 +
: 70% base sour beer
 +
: 20 g/L sucrose
 +
: 0.2 g/L Fermaid
 +
</blockquote>
 +
 +
<blockquote style="background-color: lightgrey; border: solid thin grey; padding:10px;">
 +
'''Homebrew/Low Tech Acclimation Method'''
 +
 +
This method was adapted from the Escarpment Labs and the Rogers et al. methods using non-laboratory processes and ingredients. 
 +
: ''This method has not yet been validated as far as producing the correct pitching rate for re-yeasting, which is suggested to be 1 million cells per mL of beer.''
 +
 +
For ~20 liters (or around 5-6 gallons) of sour beer:
 +
 +
# Measure 1-2 grams of dried yeast, and re-hydrate in a little bit of warm water.
 +
# Measure 50 mL of the sour beer that is going to be carbonated, and combine it with 100 mL of apple juice with no preservatives (or use DME if using a beer yeast strain) and a pinch of [http://www.scottlab.com/product-103.aspx Go-Ferm] in a saucepan.
 +
# Heat pasteurize the sour beer, apple juice, and [http://www.scottlab.com/product-103.aspx Go-Ferm] mixture on the stove at 160°F for 10 minutes. 
 +
# Cool the sour beer and apple juice mixture to around 86°F (30°C).  Pitch into an Erlenmeyer flask, along with the re-hydrated wine yeast.
 +
# Grow for ~24 hours without shaking at around 86°F (30°C) *.
 +
# Measure 200 mL of sour beer that is going to be carbonated, and combine with 200 mL of apple juice and a pinch of [http://www.scottlab.com/product-103.aspx Go-Ferm] in a saucepan.
 +
# Heat pasteurize the sour beer, apple juice (make sure there are no preservatives in the apple juice), and [http://www.scottlab.com/product-103.aspx Go-Ferm] mixture on the stove at 160°F for 10 minutes. 
 +
# Cool the sour beer and apple juice mixture to around 86°F (30°C).  Top up the Erlenmeyer flask with the yeast starter in it with this fresh mixture.
 +
# Grow for ~24 hours without shaking at around 86°F (30°C) *.   
 +
# Continue to step up the starter as needed for larger batches. 
 +
# After the 24 hours of growth is finished for the final step, add ~200 mL of the starter per 20 liters of beer (stir the starter before adding into the beer; this does not account for the sugar that needs to be added to achieve carbonation because the apple juice should be fully fermented at this stage).
 +
 +
<nowiki>*</nowiki> If a fermentation temperature of around 86°F (30°C) cannot be maintained and the temperature falls during the 24 hour growth period, allow the final step to ferment for 48 hours before adding it to the beer just to make sure the apple juice is fully fermented.
 +
</blockquote>
  
 
===Priming===
 
===Priming===
(in progress)
+
If your beer is at final gravity, you can prime it as you would any normal beer based on the amount carbonation you want. In order to properly prime your beer, you will need to know your residual CO2 in solution. This can be determined by temperature and good priming sugar calculators take this into account. For aged beers, especially beers which have been aged in barrels or a similar porous vessel, the starting CO2 may be lower than the numbers produced from these calculators due to off-gassing while the beer is aging.  We recommend using [http://jeffreycrane.blogspot.com/2015/06/blending-calculator-ph-abv-and.html Jeff Crane's "Blending Calculator" (extension of Michael Tonsmeire's "Blending Calculator")] for priming calculations with aged beer.
 +
 
 +
Tomme Arthur from The Lost Abbey reports that the starting values he sees in barrel aged beers range from 0.2-0.6 volumes of CO2<ref name='Tomme on the Sour Hour'>[http://www.thebrewingnetwork.com/the-sour-hour-the-lost-abbey/ Tomme Arthur from the Lost Abbey on the Sour Hour]</ref> (~51 minutes in). Brewers may choose to partially force carbonate their beers to know the starting CO2 exactly as well as limit the CO2 required from bottle conditioning. The Lost Abbey carbonates to 1.5 volumes of CO2 before priming and bottle conditioning for the remaining carbonation<ref name='Tomme on the Sour Hour'/> (~52 minutes in).  Alternatively, a product used in measuring residual CO2 for champagne called a [https://www.reddit.com/r/TheBrewery/comments/6ba8i8/using_a_carbodoseur_to_figure_out_co2_in_solution/ Carbodoseur] can be used to estimate residual CO2 after long aging.  See [https://www.facebook.com/groups/MilkTheFunk/search/?query=carbodoseur these MTF threads] on experiences using the Carbodoseur and the [http://www.allafrance.com/notices_pdf/99200-va_FR.pdf Carbodoseur calibration table].  If no effort is made to determine residual CO2, 0.4 has been quoted as a good estimation for aged beer <ref>[https://books.google.com/books?id=l5UIBAAAQBAJ&pg=PA271&lpg=PA271&dq=michael+tonsmeire+residual+co2&source=bl&ots=IQm2_NOAYC&sig=PuhumzttcB_qQHLmNzzGcnmIzDg&hl=en&sa=X&ved=0ahUKEwi_49e-tKDUAhUH6GMKHVBqDj4Q6AEIPzAE#v=onepage&q=michael%20tonsmeire%20residual%20co2&f=false Tonsmeire, Michael.  American Sour Beer: Innovative Techniques for Mixed Fermentations.  Brewers Publications.  06/15/2014. ISBN-13:9781938469138. Pg 271.]</ref>. 
 +
 
 +
If your beer is not at final gravity, but you have a good idea of where it will finish, then you can prime taking into account remaining gravity points.  See [[Packaging#Final_Gravity|Final Gravity]] for more information.
 +
 
 +
Alternatively, beer can be kegged and force carbonated like any other beer.  Beer can be then bottle/crowler/can filled from the keg using a beer gun or some other device.  Anecdotes from some brewers have reported a higher chance of [[Tetrahydropyridine|THP]] production when force carbonating some mixed fermentation beers (see the [[Tetrahydropyridine|THP]] page for more information).  See [[Packaging#Package_and_closure_types|Package Types]] below for more information on kegging in general.
  
In order to properly prime your beer you will need to know your residual CO2 in solution. This can be determined from temperature and good priming sugar calculators take this into account. For aged beers, especially beers which have been aged in barrels or a similar porous vessel, the starting CO2 may be lower than the numbers produced from these calculators due to off gassing while the beer is aging. Tomme Arthur from The Lost Abbey reports that the starting values he sees in barrel aged beers range from 0.2-0.6 volumes of CO2<ref name='Tomme on the Sour Hour'>[http://www.thebrewingnetwork.com/the-sour-hour-the-lost-abbey/ Tomme Arthur from the Lost Abbey on the Sour Hour]</ref> (~51 minutes in). Brewers may choose to partially force carbonate their beers to know the starting CO2 exactly as well as limit the CO2 required from bottle conditioning. The Lost Abbey carbonates to 1.5 volumes of CO2 before priming and bottle conditioning for the remaining carbonation<ref name='Tomme on the Sour Hour'/> (~52 minutes in).
+
CO2 levels in bottles can be measured with special equipment. For example, Allagash uses a [https://foodandbeverage.pentair.com/en/case-studies/allagash Haffmans CO2-Selector]. The pierce model Zahm can also be used to measure packaged CO2 levels (see [https://www.facebook.com/groups/MilkTheFunk/permalink/2210596275635241/?comment_id=2210635192298016&comment_tracking=%7B%22tn%22%3A%22R2%22%7D this MTF thread]).
  
Priming with gravity points----- to do.
+
See also:
See [http://jeffreycrane.blogspot.com/2015/06/blending-calculator-ph-abv-and.html Jeff Crane's "Blending Calculator" (extension of Michael Tonsmeire's "Blending Calculator")] for a re-yeasting and priming calculator.
+
* [https://www.facebook.com/groups/MilkTheFunk/posts/6839602712734551/ MTF thread about calculating and priming with honey.]
  
 
====Bob Sylvester's Priming Guide====
 
====Bob Sylvester's Priming Guide====
[http://saintsomewherebrewing.com/ Bob Sylvester from Saint Somewhwere Brewery] describes his method to bottle conditioning saison/farmhouse style beers on a commercial scale (amounts are for a 7 barrel/217 gallon batch of beer) <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1154486281246251/?comment_id=1154705631224316&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with Bob Sylvester on his bottle conditioning methods on MTF.  09/26/2015.]</ref>.  This method achieves high carbonation levels that are appropriate for saison and [[lambic]] styles:
+
[http://saintsomewherebrewing.com/ Bob Sylvester from Brasserie Saint Somewhere] describes his method to bottle conditioning saison/farmhouse style beers on a commercial scale (amounts are for a 7 barrel/217 gallon batch of beer) <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1154486281246251/?comment_id=1154705631224316&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with Bob Sylvester on his bottle conditioning methods on MTF.  09/26/2015.]</ref>.  This method achieves high carbonation levels that are appropriate for saison styles, which targets about 2.5 volumes and is low enough to account for some minor attenuation in the bottle <ref>[https://www.brewersfriend.com/beer-priming-calculator/ Brewers Friend's priming calculator.]</ref>.  The sugar levels listed assume the beer is not barrel aged and has residual volumes of CO<sup>2</sup> (approximately 0.86) <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1462140453814164/?comment_id=1463189457042597&reply_comment_id=1463299223698287&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Discussion on MTF with Bob Sylvester, clarifying residual CO2 levels in his finished beer.  11/07/2016.]</ref>:
  
 
# All my beers have a final gravity of pretty much zero. I use plain cane sugar. I've found it to be pretty yeast friendly and the results are faster with sucrose. Not sure why dextrose became popular. Most of us don't brew with corn, so why add it at packaging.  
 
# All my beers have a final gravity of pretty much zero. I use plain cane sugar. I've found it to be pretty yeast friendly and the results are faster with sucrose. Not sure why dextrose became popular. Most of us don't brew with corn, so why add it at packaging.  
# Use dry champagne yeast or [http://www.scottlab.com/product-57.aspx EDM wine yeast].
+
# Bob prefers Red Star brand dry champagne yeast.  Suggested alternatives include [http://www.scottlab.com/product-57.aspx EDM wine yeast], Maurivin PDM, and Fermentis Safoeno VR 44 <ref name="Gus_Sylvester_Method">[http://thefarmhouseobsession.blogspot.com/2016/06/kathleen-round-3-more-hops.html "Kathleen Round 3 - More Hops!"  The Farmhouse Obsession Blog by Andrew "Gus" Addkison. 06/08/2016.  Retrieved 06/08/2016.]</ref>.
# For 7 barrels/217 gallons of beer, re-hydrate 250 grams of yeast in 3.5 gallons of water with 12 pounds of cane sugar dissolved at 108°F.  I do this first and let it bloom while I transfer the beer from the fermenter to my kettle. Takes about 20 minutes. By this time the yeast is about to crawl out of the bucket.
+
# For 7 barrels/217 gallons of beer, dissolve the 12 pounds of cane sugar (0.88 ounces of sugar per 1 gallon of beer) in ~3.5 gallons of 108-110°F water.  After the sugar dissolves, add 250 grams of yeast to the sugar water (1.15 grams of yeast and 0.885 oz of sucrose dissolved in 2 fl oz of water per gallon of beer, or 6.628 grams of sucrose per liter of beer).  The amount of yeast is less important than the amount of sugar <ref name="Gus_Sylvester_Method"></ref>)<ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1917477671613771/?comment_id=1917618818266323&reply_comment_id=1917621641599374&comment_tracking=%7B%22tn%22%3A%22R2%22%7D Bob Sylvester on rehydrating yeast and sugar together.  Milk The Funk Facebook group.  12/13/2017.]</ref>.  I do this first and let it bloom while I transfer the beer from the fermenter to my kettle. Takes about 20-30 minutes. By this time the yeast is about to crawl out of the bucket. ''Editor's note: many homebrewers have had success carbonating sour beer with only 1-2 grams of wine yeast for 5 gallons of beer.''
# Add the yeast slurry just before transfer is finished as this will help incorporate the slurry. Carbonation happens the same day as packaging if done correctly.
+
# Add the yeast/sugar slurry just before the transfer is finished as this will help incorporate the slurry. Carbonation happens the same day as packaging if done correctly.
 
# Sounds counter to everything you've learned, but don't gas purge the bottles. The yeast will uptake the O2 in the head space.  
 
# Sounds counter to everything you've learned, but don't gas purge the bottles. The yeast will uptake the O2 in the head space.  
# Keg conditioning the same exact process but back the sugar down to 8 pounds and keep the yeast at 250 grams.
+
# Sanke keg conditioning uses the same exact process but back the sugar down to 8 pounds (0.59 ounces of sucrose per gallon of beer or 4.4186 grams of sucrose per liter of beer) and keep the yeast at 250 grams.  Fill the keg to the shoulder where the side and the lid meet (too much headspace leads to too high of a carbonation level). One recommendation for getting the right headspace is to lift one side of the keg onto a 2x4 wood block and fill it while the keg is leaning at an angle (this method works best for half barrel kegs; smaller kegs might tip over) <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/2337168479644686/?comment_id=2337190276309173&comment_tracking=%7B%22tn%22%3A%22R3%22%7D Bob Sylvester and Allen Stone.  Milk The Funk Facebook group thread about keg conditioning and fill level.  10/26/2018.]</ref>.
 +
## Note: For a discussion on why or why not the dosage of priming sugar for kegs should be lower than for bottles, see [https://www.facebook.com/groups/MilkTheFunk/permalink/4607537139274464/?comment_id=4607551389273039&reply_comment_id=4607649122596599 this thread].
 
# Wait 10 days. That's it. With time, the initial "rough" carbonation you get in the first couple days will mellow out into the fluffy stuff.
 
# Wait 10 days. That's it. With time, the initial "rough" carbonation you get in the first couple days will mellow out into the fluffy stuff.
 +
 +
====Krausening====
 +
David Fuhrer reported using a [http://brewwiki.com/index.php/Speise "speise"] method similar to krausening for mixed fermentation beers reduced the time of [[Tetrahydropyridine|THP]] in mixed fermentation bottles to 3 weeks. See [https://www.masterbrewerspodcast.com/237 Episode 237 of the MBAA podcast] and related links.
 +
 +
See also:
 +
* [http://beersmith.com/blog/2010/03/22/krausening-home-brewed-beer/ BeerSmith blog article on krausening calculations.]
  
 
===Oxygen exposure===
 
===Oxygen exposure===
Oxygen is the enemy of finished beer, and most producers of standard beers invest a lot of time and money to reduce oxygen exposure in their finished beer. For mixed fermentation/sour/funky beers, this may not be as significant a concern. These beers, especially barrel aged versions, generally have more oxygen exposure during fermentation and aging and this contributes to the characteristics of the beer. Some brewers achieve good results without purging bottles before filling (for example, lambic producers and blenders), and others do take care to avoid oxygen exposure in the packaging process. There is no definite answer in terms of whether it is better to carefully CO2 purge as is recommended in more normal beers or if the yeasts present in mixed-fermentation beers are better able to quickly scavenge O2 before it can damage the beer, though excessive oxygen exposure can hurt your beer (either permanently through formation of acetic acid and/or ethyl acetate, or temporarily by influencing [[tetrahydropyridine]] (THP). Based on your experience, equipment, and preferences, you can determine how much to limit O2 exposure in your beers. The safest route is to treat them as you would a normal beer and take care to avoid O2, though the experience of some will say that this extra work and cost is not necessary.
+
Oxygen is the enemy of finished beer, and most producers of standard beers invest a lot of time and money into reducing oxygen exposure in their finished beer (referred to as "dissolved oxygen" for oxygen in the beer or "total packaged oxygen" for total oxygen in the beer plus additional oxygen in the headspace of the package).  Excessive oxygen exposure can negatively impact the beer either permanently through the formation of acetic acid and/or ethyl acetate, temporarily by increasing [[tetrahydropyridine]] (THP) formation, or through the [[Aging_and_Storage#General_Effects_of_Oxidation|oxidation of various compounds]]. For mixed fermentation beers where fresh bottling yeast is added, this may not be as significant of a concern, depending on how much oxygen is allowed to enter the packaging. These beers, especially barrel aged versions, generally have more oxygen exposure during fermentation and aging, and this contributes to the characteristics of the beer. ''Brettanomyces'' is known as an "oxygen scavenger", which helps to prevent oxidative reactions in the bottle over time (see [[Aging and Storage]]).  Fresh bottle conditioning yeast can help prevent acetic acid and THP production by consuming the oxygen in non-purged bottles before it can have an effect on the mixed culture.  Even without fresh bottling yeast, some brewers achieve good results without purging bottles before filling (for example, lambic producers and blenders), although this might be dependent on whether the strains present in the beer are big THP producers or if the brewer has limited time to store bottles long enough for the THP to age out.  Others do take care to avoid oxygen exposure in the packaging process and report that purging bottles with CO<sub>2</sub> helps prevent THP formation. Due to these many variables, there is no easy answer in terms of whether it is better to carefully CO<sub>2</sub> purge as is standard practice for clean beers.  CO<sub>2</sub> purging can require more expensive equipment, as well as require more time to package. Based on the characteristics of the microbes being used, experience, equipment, process, and preferences, the brewer can determine how much limiting O<sup>2</sup> exposure during packaging is necessary. The safest route is to treat them as you would a normal beer and take care to avoid O<sup>2</sup> where this is possible or affordable, though the experience of some will say that this extra work and cost might not be completely necessary like it is for clean beers. 
 +
 
 +
If force carbonating the beer instead of bottle conditioning with fresh yeast, or if bottle conditioning without adding fresh yeast, it might be best practice to purge bottles and kegs with CO<sup>2</sup> in order to limit the production of both acetic acid and THP.  Again, experience should drive this decision one way or the other.
 +
 
 +
See also:
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/2375853352442865/ MTF thread on whether or not to purge the top of the bottling machine with CO<sub>2</sub>.]
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/2282287018466166/ Justin Amaral's can conditioning project with DO tracking for canned conditioned beers on MTF.]
 +
* [https://www.facebook.com/groups/MilkTheFunk/posts/6845568052138017/ MTF thread on the science available regarding the idea that yeast can scavenge oxygen after packaging.]
 +
 
 +
MBAA podcasts:
 +
* [https://www.masterbrewerspodcast.com/055 Episode 50 - TPO with Kevin Sudderth & Mike Feldman from Hach.]
 +
* [https://www.masterbrewerspodcast.com/150 Episode 150 - TPO Gone Wild? Managing Oxygen Ingress on Smaller Scale Canning Lines.]
 +
* [https://www.masterbrewerspodcast.com/178 Episode 178 - Friends Don't Let Friends Drink Oxidized Beer (minimizing oxygen in brite tanks and packaging with guest Audrey Skinner).]
  
 
===Package and closure types===
 
===Package and closure types===
 
(in progress)
 
(in progress)
 
Many sour/funky/mixed fermentation beers are highly carbonated. It is recommended to package highly carbonated beers in bottles of thicker glass which can better withstand higher pressure (e.g. many Belgian and German bottles, corkable Champagne-type or 'fat-lipped' Belgian-type bottles, etc.). This is especially true if you feel the beer may continue to carbonate in the bottle from residual carbohydrates beyond the priming sugar you added. Be careful when carbonating your beer to high volumes to ensure that your chosen package can handle this pressure.
 
Many sour/funky/mixed fermentation beers are highly carbonated. It is recommended to package highly carbonated beers in bottles of thicker glass which can better withstand higher pressure (e.g. many Belgian and German bottles, corkable Champagne-type or 'fat-lipped' Belgian-type bottles, etc.). This is especially true if you feel the beer may continue to carbonate in the bottle from residual carbohydrates beyond the priming sugar you added. Be careful when carbonating your beer to high volumes to ensure that your chosen package can handle this pressure.
 +
 +
For information about corking, see the [[Corking]] page.
  
 
* '''Crown caps (26 mm)''' - A variety of thicker glass/high-pressure bottles are available in the standard 26 mm cap size. No special equipment, beyond a normal capper, is necessary to fill bottles of this type. It may be necessary to swap the metal plates on cappers such as the Emily capper to accomodate the wider neck on some bottles taking a 26 mm cap (such as champagne-style bottles with 26 mm caps (e.g. Logsdon, Goose Island, some Upright) and the [http://www.northernbrewer.com/shop/375-ml-belgian-style-beer-bottles-crown-finish 375 mL crown finish bottles] modeled after the 'Vinnie' 375 mL bottles).
 
* '''Crown caps (26 mm)''' - A variety of thicker glass/high-pressure bottles are available in the standard 26 mm cap size. No special equipment, beyond a normal capper, is necessary to fill bottles of this type. It may be necessary to swap the metal plates on cappers such as the Emily capper to accomodate the wider neck on some bottles taking a 26 mm cap (such as champagne-style bottles with 26 mm caps (e.g. Logsdon, Goose Island, some Upright) and the [http://www.northernbrewer.com/shop/375-ml-belgian-style-beer-bottles-crown-finish 375 mL crown finish bottles] modeled after the 'Vinnie' 375 mL bottles).
 
* '''Crown caps (29 mm)''' - For capping bottles with 29 mm openings (such as Champagne-style bottles and 375 mL half-Champagne bottles), you will need to source a 29 mm bell and 29 mm caps, which are non-standard. Many 29 mm bottles are both capable and corkable. For cork and capping, see below.
 
* '''Crown caps (29 mm)''' - For capping bottles with 29 mm openings (such as Champagne-style bottles and 375 mL half-Champagne bottles), you will need to source a 29 mm bell and 29 mm caps, which are non-standard. Many 29 mm bottles are both capable and corkable. For cork and capping, see below.
 
* '''Swing tops''' - Many swing tops are thicker glass and are therefore well suited for higher carbonation beers. Swing tops also allow for easy venting of carbonation if the beer carbonates more than desired/anticipated. Due to the soft gasket (and possibly plastic top piece holding the gasket for some bottles), the same concerns regarding cleaning difficulty and cross-contamination apply to swing tops as to other plastic parts used after the hot side when brewing both normal and 'funky' beers. If you don't want your normal beers exposed to the micro-organisms in your funky beers, we recommend not using the same swing tops for bottling both types of beer, jusrt as we recommend keeping seperate sets of plastic equipment used for fermentation and transfer of fermented beer when brewing both clean and 'funky' beers. Some express concern about long term aging in swing tops and the possibility of swing tops not being as impermeable a barrier to O2. If you are concerned about this they it may be better to not use swing tops for long aged beer. At this time we are unaware of any comparisons of swing tops and other closure mechanisms regarding their susceptibility to O2 transfer during extended aging to give a firm answer as to whether swing tops allow more O2 ingress than other closures or not .
 
* '''Swing tops''' - Many swing tops are thicker glass and are therefore well suited for higher carbonation beers. Swing tops also allow for easy venting of carbonation if the beer carbonates more than desired/anticipated. Due to the soft gasket (and possibly plastic top piece holding the gasket for some bottles), the same concerns regarding cleaning difficulty and cross-contamination apply to swing tops as to other plastic parts used after the hot side when brewing both normal and 'funky' beers. If you don't want your normal beers exposed to the micro-organisms in your funky beers, we recommend not using the same swing tops for bottling both types of beer, jusrt as we recommend keeping seperate sets of plastic equipment used for fermentation and transfer of fermented beer when brewing both clean and 'funky' beers. Some express concern about long term aging in swing tops and the possibility of swing tops not being as impermeable a barrier to O2. If you are concerned about this they it may be better to not use swing tops for long aged beer. At this time we are unaware of any comparisons of swing tops and other closure mechanisms regarding their susceptibility to O2 transfer during extended aging to give a firm answer as to whether swing tops allow more O2 ingress than other closures or not .
* '''Cork and cage''' - Corking and caging can be used for both champagne-type bottles and the brown glass 'fat-lipped' Belgian bottles, both of which are generally thicker glass and rated for higher CO2 pressure. Generally corking and caging is used for bottles with 29 mm openings, though some have cork and cage finished bottles with 26 mm openings. Make sure your bottle can take corks before using it. Corking and caging does require some more special equipment. Most wine corkers are designed to push the cork all the way into the bottle, which is not what you want. Brewers will at least want a [[Floor Corker|floor corker]] for standard wine bottling, which can be modified to control the cork depth. Bench corkers such as the Colonna capper/corker work as well. Champagne floor corkers are available and make this easier, though they are more expensive. It is difficult to control the exact depth of the cork with a two arm corker, and we do not recommend this for corking and caging. The following gives a step by step process for corking and caging with a non-champagne floor corker. For a more detailed description with pictures, see [http://horscategoriebrewing.blogspot.com/2013/11/corking-your-homebrew.html Dave Janssen's blog post about corking]. The more common floor corkers work by both compressing the cork and pushing it into the bottle with the movement of the arm. In order to leave the cork partly exposed, and to control the exposed cork level, place a standard carboy bung on the rod which pushes the cork. This positioning can be adjusted as needed. Push the cork into the bottle until you reach the bung, then bring the arm all the way up. Remove the bung and lower the spring-loaded pedestal holding the bottle. Then, while holding the bottle with one hand, lower the corking arm completely and bring the bottle down, letting the cork be pushed fully out of the compression section of the corker. Play around with cork depth to find the right amount for you for ease of removal based on your corks, bottles and carbonation levels. The amount of cork sticking out should be more than how it appears when it is caged, as the cork is vertically compressed or 'mushroomed' during the caging process. This mushrooming can be accomplished by hand, though be aware that flaws in the bottle may cause the neck to break, which would be a very dangerous situation if you are applying the downward force by your hand. It can more easily and safely be applied with a bench capper. Put the cage on the cork and compress both with a bench capper. Hold the bench capper arm in place with your shoulder/armpit, leaving both hands free to orient and twist the cage. Special cage twisting tools are available, though sharpies or some interchangeable screwdrivers are about the right size. You could also use twist lock pliers if you have them around.
+
* '''Kegging''' - Kegging offers the benefit of being able to force carbonate or naturally carbonate with adding priming sugar and (optionally) re-yeasting.  If force carbonating, follow the same procedures that you would for any other beer.  Consider the [https://www.reddit.com/r/Homebrewing/comments/hfzuh/how_to_properly_carbonate_your_homebrew_in_kegs/ "set and forget"] method of force carbonating, which requires 1-3 weeks.  This will allow the beer to settle while also dialing in the carbonation level.  Priming in the keg often produces great results with sour beers.  Generally, use 1/2 of the priming sugar that you would normally use, or excessive foaming can occur <ref>[https://byo.com/hops/item/954-keg-your-beers Don Million.  Keg Your Beers! Brew Your Own Magazine. 2003.]</ref>.  If re-yeasting, which is generally recommended (see the [[Packaging#Re-yeasting|Re-yeasting]] section above), use 10% of the yeast you would normally use to for a primary fermentation.  For example, use around 2 grams of dried champagne yeast re-hydrated to naturally carbonate 5 gallons of beer <ref name="priming_calc"></ref>.  Priming in the keg will result in a little more sediment at the bottom of the keg, but this will be pulled out on the first pour from the keg.  Allow at least three weeks of conditioning at room temperature, and then a week at refrigeration temperatures before serving.  If the keg is not re-yeasted, it might take considerably longer than three weeks to carbonate the beer depending on the vitality of the yeast in the beer.  The brewer should consider keeping plastic kegging equipment such as serving lines and taps separate from kegs that serve clean beers.  
* '''Cork and cap''' - Corking for cork and cap closures can also be used on champagne-style bottles, which are a good source of high-pressure bottles for brewers. Corking and capping is much more straightforward as the cork is pushed entirely into the bottle. Generally cork and cap closures are used on bottles with 29 mm openings, though some have done this with 26 mm bottles. Make sure your bottle can handle corks before using them for a cork and cap finish. Although the cork depth is not as important as in corking and caging, the cork still must be far enough into the bottle to not interfere with the cap. Bench corkers are recommended for corking and capping as they allow better control of cork depth than two arm corkers and bench corkers allow the cork to be easily depressed further if it is not far enough into the bottle. For 29 mm bottles, you will also need a 29 mm bell and 29 mm caps for capping.
+
 
* '''Kegging''' - Kegging offers the benefit of being able to force carbonate or naturally carbonate with adding priming sugar and (optionally) re-yeasting.  If force carbonating, follow the same procedures that you would for any other beer.  Consider the [https://www.reddit.com/r/Homebrewing/comments/hfzuh/how_to_properly_carbonate_your_homebrew_in_kegs/ "set and forget"] method of force carbonating, which requires 1-3 weeks.  This will allow the beer to settle while also dialing in the carbonation level.  Priming in the keg often produces great results with sour beers.  Generally, use 1/2 of the priming sugar that you would normally use, or excessive foaming can occur <ref>[https://byo.com/hops/item/954-keg-your-beers Don Million.  Keg Your Beers! Brew Your Own Magazine. 2003.]</ref>.  If re-yeasting, which is generally recommended (see the [[Packaging#Re-yeasting|Re-yeasting]] section above), use 10% of the yeast you would normally use to for a primary fermentation.  For example, use around 2 grams of dried champagne yeast re-hydrated to naturally carbonate 5 gallons of beer <ref name="priming_calc"></ref>.  Priming in the keg will result in a little more sediment at the bottom of the keg, but this will be pulled out on the first pour from the keg.  Allow at least three weeks of conditioning at room temperature, and then a week at refrigeration temperatures before serving.  If the keg is not re-yeasted, it might take considerably longer than three weeks to carbonate the beer depending on the vitality of the yeast in the beer.  The brewer should consider keeping plastic kegging equipment such as serving lines and taps separate from kegs that serve clean beers.  
+
:A [http://seanterrill.com/2015/06/25/build-a-better-spunding-valve/ spunding valve] can also be used to help guard against over-carbonation when keg conditioning.  The CO2 gauges on spunding valves can be inaccurate, however, so finding the correct PSI setting requires the following technique.  The goal is to set it to be around 30 PSI for room temperature conditioning.  First, pressurize the keg to 30 PSI using a reliable CO2 regulator, then remove the gas line while the keg is still pressurized.  Fully tighten the spunding valve's pressure release valve ('''PRV''') so that no gas is released, and attach it to the keg's gas line.  Slowly turn the PRV on the spunding valve so that the gas is released.  Continue to release the gas until the faintest "hiss" of gas can be heard, and then slightly tighten the PRV.  The PRV might be set to something like 40 or 50 PSI, but the reliable CO2 regulator can be used to verify that the spunding valve's PRV is set to the desired level.  The actual PSI within the keg might oscillate around 27-32 PSI, so this technique might need some trial and error.  Shoot for under-carbonation versus over-carbonation.  If the beer is slightly under-carbonated, force carbonation can make up the difference.  Slight over-carbonation should be avoided because it could make the beer very difficult to serve <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1373107886050755/?comment_id=1374126712615539&reply_comment_id=1374144919280385&comment_tracking=%7B%22tn%22%3A%22R3%22%7D Keg conditioning thread on MTF with Bryan from Sui Generis and Devin Henry.  08/12/2016.]</ref>. 
* '''Green glass v Brown glass''' - Some bottle types, especially champagne style bottles, may be more available in green glass compared to brown glass. Green glass does a worse job shielding the beer from UV spectrum light, which can interact with hop compounds to produce 'skunky' or lightstruck flavors and aromas. Many classic Belgian mixed fermentation beers are found in green glass, and some producers in North America seek out green glass over brown glass for their beers<ref>[http://jesterkingbrewery.com/jester-king-in-green-bottles Jester King blog post about green bottles]</ref><ref>[http://www.thebrewingnetwork.com/the-sour-hour-episode-15/ Jester King on the Sour Hour, pt 2]</ref> (~11 minutes in) (also, Bob Sylvester).
+
:Another method for setting the spunding valve's PRV to the correct PSI setting would be to use a keg of beer that is already perfectly carbonated.  Allow the keg to warm to conditioning temperatures.  Attach the spunding valve with the PRV completely closed.  Slowly release the PRV until you hear a slight hiss.  This should reach the PSI setting that the spunding valve needs to be set at to reach around the same carbonation for other kegs.  Kegs should be filled up to the same headspace as the keg that was used to set the PRV setting <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1462140453814164/?comment_id=1462348370460039&reply_comment_id=1462389737122569&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Keg conditioning thread on MTF with Taylor John Caron.  11/07/2016.]</ref>.
 +
 
 +
* '''Green glass v Brown glass''' - Some bottle types, especially champagne style bottles, may be more available in green glass compared to brown glass. Green glass does a worse job shielding the beer from UV spectrum light, which can interact with hop compounds to produce 'skunky' or lightstruck flavors and aromas. Many classic Belgian mixed fermentation beers are found in green glass, and some producers in North America seek out green glass over brown glass for their beers <ref>[http://jesterkingbrewery.com/jester-king-in-green-bottles Jester King blog post about green bottles]</ref><ref>[http://www.thebrewingnetwork.com/the-sour-hour-episode-15/ Jester King on the Sour Hour, pt 2]</ref> (~11 minutes in) (also, Bob Sylvester). See also [[Aging_and_Storage#Lightstruck|Lightstruck]].
 +
 
 
* '''Crown caps v corks''' - Deciding between corks and caps will depend on the specific beer and the brewer's goals with the beer. Caps are a cheaper closure and are also easier to apply. Aside from aesthetics, you may choose corks when intending to age or store beers lying down in order to keep the beer from interacting with the cap. Some producers have observed that crown caps contribute to an off-flavor in their beer, especially if the beer is acidic. Pierre Tilquin closes some bottles of every blend with crown caps in order to test carbonation levels. He has noticed that the crown capped bottles have an off flavor not present in the corked bottles <ref>Conversation between Pierre Tilquin and Dave Janssen, 24-Oct-2015</ref>. Other producers regularly package their acidic beer with crown caps (e.g. the Rare Barrel, Boon Geuze in 25 cl bottles) and have not mentioned a problem with doing so.
 
* '''Crown caps v corks''' - Deciding between corks and caps will depend on the specific beer and the brewer's goals with the beer. Caps are a cheaper closure and are also easier to apply. Aside from aesthetics, you may choose corks when intending to age or store beers lying down in order to keep the beer from interacting with the cap. Some producers have observed that crown caps contribute to an off-flavor in their beer, especially if the beer is acidic. Pierre Tilquin closes some bottles of every blend with crown caps in order to test carbonation levels. He has noticed that the crown capped bottles have an off flavor not present in the corked bottles <ref>Conversation between Pierre Tilquin and Dave Janssen, 24-Oct-2015</ref>. Other producers regularly package their acidic beer with crown caps (e.g. the Rare Barrel, Boon Geuze in 25 cl bottles) and have not mentioned a problem with doing so.
* '''Large format bottles''' - To cork large format bottles (1.5 L and up) without specialized equipment for large format corking, you will have to modify a corking process and/or possibly create some new equipment. MTFers have found that when a bottle does not fit into a corker, the corks can be inserted by compression the cork in a corker and then ejecting it and quickly inserting the cork into the bottle<ref name='MTF Large Format Corking'>[https://www.facebook.com/groups/MilkTheFunk/permalink/1207333129294899/ MTF Discussion on corking large format bottles]</ref>. Raf has had success with a machined piece of wood and hammering the cork in and then mushrooming with a drill press<ref name='MTF Large Format Corking'/>. Hand corkers may also work but with them it is difficult to control cork depth. Vinnie from Russian River reports mushrooming the corks with  2x4 and his body weight<ref>[http://www.thebrewingnetwork.com/post1835/ Vinnie Cilurzo on the Brewing Network 15-Jan-2006]</ref> (1:22:30 in). 75 cl and 150 cl bottle have the same size opening and 3-9 l bottles have a larger, but consistent, opening. Some MTFers have used normal beer corks in large format bottles while others recommend sourcing larger corks<ref name='MTF Large Format Corking'/>.
 
  
==Bottle Suppliers==
+
===Corking===
===Single Cases===
+
See [[Corking]] and [[Floor Corker]] fore more information on corking and using floor corkers.
* [http://www.shorecontainer.com/750mlamberchampagneflatbottom.aspx Shore Container, 750 mL 26 mm cap bottles.]
+
 
===Bulk===
+
===Canning===
 +
Usually, only pasteurized sour beer is canned due to the danger of over-carbonating canned beer, however, some brewers have been experimenting with canning mixed fermentation beer and allowing the beer to naturally carbonate in the cans.  It is recommended that brewers contact their can supplier to verify that the plastic liners for the cans are rated to handle the [[Titratable Acidity]] of sour beer (kettle soured or otherwise) and to find out the lifespan of the cans. Cans have been found to preserve some hop compounds such as myrcene and caryophyllene better than bottles because bottle caps can strip some of these compounds <ref>[https://onlinelibrary.wiley.com/doi/full/10.1002/jib.667 Kemp, O., Hofmann, S., Braumann, I., Jensen, S., Fenton, A., and Oladokun, O. (2021) Changes in key hop-derived compounds and their impact on perceived dry-hop flavour in beers after storage at cold and ambient temperature. J. Inst. Brew., https://doi.org/10.1002/jib.667.]</ref>.
 +
 
 +
One study by [https://www.tandfonline.com/doi/pdf/10.1080/03610470.2024.2395677 Sheehan et al. (2024)] examined 9 different sour beers canned with 4 different liners with their maximum thickness available from the manufacturer: BPA based epoxy, acrylic, and two versions of BPA-NI epoxies. They aged the cans up to 48 weeks at 20°C. Out of the 9 different sour beers examined, they found that one sour beer with 7.55 g/L lactic acid (the other sour beers that did not heavily erode the liners had 5.84 g/L or less lactic acid) and one sour beer with 9.01 g/L acetic acid eroded the liners and increased the dissolved aluminum to elevated levels of 9.3 mg/L and 43.2 mg/L. This occurred at the 48 week mark. The European Food Safety Authority advises consumption of no more than 1 mg/kg body weight per week; the World Health Organization advises a limit of 2 mg/kg of body weight per week. Aluminum is regularly used as a food additive in small amounts, and most aluminum consumed is filtered by the kidneys. Regular exceedence of the weekly intake can increase the risk of health impairments, including developmental toxicity, neurotoxicity, and effects on the urinary tract and potentially the digestive track. There is no consistent evidence linking aluminum exposure from food and water to Alzheimer's disease or breast cancer. All 4 liner types were eroded by the two beers and did not comparatively protect the beers from aluminum contamination. For the beer with high acetic acid, which was deemed not sellable by the brewery that produced it and dumped, consumption of a 355 mL (12 oz) serving per day would equate to ~15 mg/day aluminum, or ~100 mg/week. This value approaches the World Health Organization recommended total weekly intake of <2 mg Al/week per kg body weight, or <150 mg Al/week for a 75 kg person. Can erosion was not correlated with pH, but it was correlated with [[Titratable Acidity]]. It is recommended that breweries send samples of their beer to their can supplier to have the beer tested to make sure that the liner will not erode <ref>[https://www.tandfonline.com/doi/pdf/10.1080/03610470.2024.2395677 Matthew Sheehan, Austin Montgomery, Julie M. Goddard & Gavin L. Sacks (15 Oct 2024): Lactic and Acetic Acids in Sour Beers Promote Corrosion During Aluminum Beverage Can Storage, Journal of the American Society of Brewing Chemists, DOI: 10.1080/03610470.2024.2395677.]</ref><ref>[https://pmc.ncbi.nlm.nih.gov/articles/PMC3998638/ Pineton de Chambrun G, Body-Malapel M, Frey-Wagner I, Djouina M, Deknuydt F, Atrott K, Esquerre N, Altare F, Neut C, Arrieta MC, Kanneganti TD, Rogler G, Colombel JF, Cortot A, Desreumaux P, Vignal C. Aluminum enhances inflammation and decreases mucosal healing in experimental colitis in mice. Mucosal Immunol. 2014 May;7(3):589-601. doi: 10.1038/mi.2013.78. Epub 2013 Oct 16. PMID: 24129165; PMCID: PMC3998638.]</ref><ref>[https://www.sciencedirect.com/science/article/abs/pii/S0045653521030289#:~:text=Aluminum%20exposure%20can%20damage%20the,stability%20of%20the%20biological%20barrier Wudi Hao, Chenyu Hao, Chengrong Wu, Yuqing Xu, Cuihong Jin,Aluminum induced intestinal dysfunction via mechanical, immune, chemical and biological barriers, Chemosphere. Volume 288, Part 2, 2022, 132556, ISSN 0045-6535. https://doi.org/10.1016/j.chemosphere.2021.132556.]</ref>. See also [https://www.facebook.com/groups/MilkTheFunk/posts/9393681103993353/ this post on MTF].
 +
 
 +
See also:
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/2655248284503369/ General MTF tips on canning]. 
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/2705352459492951/ Discussion on the safety of canning mixed fermentation sour beer versus Coke (pH 2.5) and kettle sours, as well as other general canning information.]
 +
* See this [https://www.facebook.com/speciationartisanales/videos/125350678350276/ video of Speciation Artisen Ales canning process (conditioning in the can)].
 +
** [https://www.facebook.com/groups/MilkTheFunk/permalink/2260040164024185/?comment_id=2260061030688765&reply_comment_id=2260181380676730&comment_tracking=%7B%22tn%22%3A%22R%22%7D Mitch Ermatinger gives advice on using cans.]
 +
** [https://www.facebook.com/groups/MilkTheFunk/permalink/2705564312805099/ More videos from Mitch on canning using a GW Kent filler and Oktober seamer.]
 +
** [https://www.facebook.com/groups/MilkTheFunk/?post_id=3803228973038622&comment_id=3803870986307754 Mitch's advice on CO<sub>2</sub> levels when can conditioning mixed fermentation sours.]
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/2282287018466166/ Justin Amaral's can conditioning project with DO tracking for canned conditioned beers on MTF.]
 +
* Mobile canning companies that will can mixed fermentation beer:
 +
** [https://craftcanning.com/ Craft Canning]
 +
* [https://www.masterbrewerspodcast.com/224 MBAA Podcast Ep 224 "Can Liner Quality & Testing".]
 +
* [https://seamschool.com/ Seam School]
 +
* [https://web.archive.org/web/20220110012615/https://doubleseam.com/ Double Seam]
 +
 
 +
===Capping===
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/2070646812963522/ Brad Primozic's video of bottling and capping sour beer for conditioning in a small commercial brewery.] 
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/2172307539464115/ Sean McVeigh's packaging process on MTF.]
 +
* See also the [[Corking]] page for more small commercial brewery packaging videos.
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/2433280286700171/?comment_id=2617756571585874&comment_tracking=%7B%22tn%22%3A%22R%22%7D MTF tips on fixing cappers that stick.]
 +
 
 +
===Using Sulfite===
 +
Sulfite is generally not added to mixed fermentation beer. It may be possible to use sulfite to inhibit refermentation of unfermented sugars. There is also some speculation that sulfite might help reduce [[Tetrahydropyridine]].
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/4069328623095321/?comment_id=4069360419758808 MTF comments by Shawn Savuto (experience using sorbate and sulfite to inhibit fermentation with resource links).] See also the [https://www.facebook.com/groups/MilkTheFunk/permalink/4435140659847447 Purdue sulfite calculations in winemaking resource] here.
 +
 
 +
==Equipment==
 +
===Bottle Suppliers===
 +
====Single Cases====
 +
* [http://www.shorecontainer.com Shore Container, 750 mL 26 mm cap bottles.]
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* [http://www.homebrewing.org Homebrewing.org; 750 mL cork and cage bottles and 375 mL cork and cage bottles.]
 +
* [https://www.morebeer.com/ MoreBeer Amber Champagne/Belgian Style bottles (also has non-punted versions).]
 +
* [https://morewinemaking.com MoreWine Champagne bottles.]
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* [http://mdhb.com Maryland Homebrew: 750 mL Belgian bottles and Champagne Bottles (offers 3 liter magnums).]
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* [https://www.curdsandwine.com/ Curds and Wine (green champagne).]
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* [http://www.keystonehomebrew.com Keystone Homebrew Supply (green bottles 750 mL).]
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* [https://www.midwestsupplies.com Midwest Supplies: 375/750 mL Belgian bottles.]
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* [http://www.northernbrewer.com Northern Brewer: 750 mL and 350 mL, cork and caged, and crown capped Belgian bottles.]
 +
* [https://www.baderbrewing.com Bader Beer and Wine Supply - 3 Liter Antique Green Flip-top bottle.]
 +
* [https://www.austinhomebrew.com Austin Homebrew Supply.]
 +
* [https://brewshop.no Brewshop, Norway: 750 mL and 375 mL Champagne bottles.]
 +
 
 +
====Bulk====
 +
* [http://www.zh-inc.com/main.php Zuckerman Honickman.]
 +
* [http://www.unitedbottles.com/ United Bottles & Packaging (has magnums).]
 +
* [https://beer.buyourbottles.com/ Buy Our Bottles.]
 +
* [https://www.wineandbeersupply.com Wine and Beer Supply.]
 +
* [https://shop.countrymalt.com/supplies-and-cooperage Country Malt Group.]
 +
* [https://www.saverglass.com/en/catalog/sparkling-wines Saver Glass (multiple sizes and styles of green glass bottles.]
 +
* [http://www.saxcodirect.com/375-ml-belgian-bottles-in-cases-crown-finish.html Saxco International.]
 +
* [http://www.waterloocontainer.com Waterloo Container.] (Also has large formats: 1.5L, 3L, 6L, and 9L, and 375 mL green bottles).
 +
* [http://landandseapackaging.com Land and Sea Packaging.]
 +
* [http://egrandstand.com/750ml-amber-belgian-beer-bottle-with-crown-finish-264427.html Grandstand Glassware & Apparel.]
 +
* [http://bsghandcraft.com/index.php/bottling-kegging-barreling/belgian-beer-bottles-corks-wires.html BSG HandCraft.]
 +
* [http://www.wiegand-glas.de/front_content.php?idcat=106&idart=3201&lang=2 Weigand Glass (750 mL, 29 mm caps or corks required).]
 +
* [http://www.hauserpack.com/catalog/beer/method-tradition/ Hauser Pack: Traditional sparkling wine green 750 mL, 29 mm caps or corks required] and [http://www.hauserpack.com/catalog/beer/champagne-ecova/?fref=gc Champagne Ecova] (and other high pressure formats).
 +
* [http://www.scottlab.com/products-4.aspx Scott Labs.]
 +
* [https://www.estal.com Estal (large formats).]
 +
* [https://pioneernorthwest.com/products/ Pioneer Packaging (mostly Champagne/wine bottles that must be corked).]
 +
* [https://allamericancontainers.com/glass-bottles-jars/ All American Containers (might have 375 mL green glass bottles).]
 +
* [http://www.stoneycreekglass.ca/ Stoney Creek Glass (Canada).]
 +
* [http://verallia.com Verallia (US and Europe).]
 +
* [http://www.wiegand-glas.de/front_content.php Weigland Glass (Germany/Europe).]
 +
* [http://aechapman.co.uk/ A.E. Chapman (UK).]
 +
* [https://www.croxsons.com/our-products/375ml-champagne-2/ Croxsons champagne bottles (multiple sized green bottles, including 375 mL and 3L - UK).]
 +
* [http://www.creopack.se/forpackningar/dryck/olflaskor/amber-champagne-design-37-5-cl Creopack (Sweden).]
 +
* [https://katalog.vetropack.com/catalogue/detail/20902#ph=1&filter_sMatAtvFuellvoll=200-412.7 Vetropack (UK).]
 +
* [http://www.vetrispeciali.com/it/prodotti/chopin Vetri Speciali green champagne bottles (It).]
 +
* [https://shop.brewcraftusa.com BrewcraftUSA (has 1.5 L/magnums).]
 +
* [https://www.keystonefermentationsupply.com/ Keystone Fermentation Supply (has magnums in green glass).]
 +
* [https://ginopinto.com/winery-commercial-catalog/ Gino Pinto Incorporated (only has 750 mL green glass with punt; 29 mm cap size).]
 +
* [https://www.exclusives.de/Glasflaschen.php Exclusives Design Glasflasch/Goseflasche/old gose style bottles (Germany).]
 +
* [https://www.wiegand-glas.de/front_content.php Wiegland-glas (Germany).]
 +
* [https://www.berlinpackaging.com/bottles/ Berlin Packaging (many sizes and types - Germany).]
 +
* [https://www.alibaba.com/showroom/500ml-green-beer-bottle.html Alibaba (green glass, may be lower quality from China).]
 +
 
 +
====Bulk 29mm Caps====
 +
* [https://www.pelliconi.com/site/en/crown-corks-29mm/ Pelliconi.]
 +
* [https://unitedbottles.com/product/29-mm-crown-cap-bc017 United Bottles.]
 +
* [https://www.gwkent.com/large-29-mm-crown-caps.html GW Kent.]
 +
* [https://bsghandcraft.com/29-mm-gold-crown-caps-bag-of-100 BSG (package of 100 caps).]
 +
* [https://www.wineandbeersupply.com/products/29mm-crown-cap?_pos=2&_sid=268b8934c&_ss=r Wine & Beer Supply.]
 +
* [https://www.waterloocontainer.com/closures/crowns/ch-crowns-vo-29mm-stainlessgold Waterloo Containers.]
 +
* [https://aowilson.ca/our-products/caps-closures/ AOWilson (Canada).]
 +
* [https://www.facebook.com/Dominion-Grimm-Inc-259502154148301/ Dominion and Grimm (Canada).]
 +
* [http://eurocapeurocork.com/ Euro Cap Cork.]
 +
* [http://www.sbi4beer.com/Products/Bottles+%26+bottling/Crown+Corks/Gold+-+29+mm/ Selected Brewing Ingredients (Netherlands).]
 +
* [https://www.thebottlejarstore.co.uk/product/sparkling-wine-29mm-crown-gold-with-bidule The Bottle Jar Store - 29mm with bidule (UK).]
 +
* [https://www.labrenta.com.br/pt/prod/tampas-para-cervejas-frisante/medusa-29 Labrenta - "Medusa" 29mm Bidule caps (BZ).]
 +
See also:
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/2595171063844425/ MTF discussion on the reliability of 29mm caps without liners.]
 +
* [https://www.facebook.com/groups/MilkTheFunk/posts/5869077499787082/ MTF discussion on the function of biduled caps.]
 +
* [https://www.facebook.com/groups/MilkTheFunk/posts/5256403844387787/ Sources for Pelliconi 29mm crowns.]
 +
 
 +
====Corkers, Corks, and Cages====
 +
See [[Corking]].
 +
 
 +
===Bottle Fillers===
 +
====Cheap and DIY====
 +
* [https://www.beer-wine.com/stainless-gravity-feed-3-spout-bottle-filler 3 Spout Bottle Filler for Commercial Use], and the [https://www.beer-wine.com/bottle-filler-5-spout-and-3-spout-gravity-feed-semiauto 5 spout version].  See also [https://www.facebook.com/groups/MilkTheFunk/permalink/1324183294276548/ this MTF thread on reviews of this product.]
 +
* [http://discussions.probrewer.com/showthread.php?26569-4-head-bottle-filler-for-275 DIY 4 Head Bottle Filler for $275 on ProBrewer.com.]
 +
* [http://vinesandrushes.com/equipment/ DIY Counter Pressure Bottler Design (scoll down).]
 +
* [https://www.homebrewersassociation.org/pimp-my-system/automatic-bottle-filling-system/?platform=hootsuite DIY 4 Head Bottle Filler from Guillaume Boulanger.]
 +
* [https://morewinemaking.com/products/wine-bottle-filler-deluxe-3-spout.html MoreWine 3 Bottle Semi-Auto Filler.]
 +
* [http://www.vigoltd.com/Catalogue/Bottle-filling/Syphon-fillers-for-beer/Stainless-steel-syphon-fillers (UK) Vigo 2 and 4 head bottle stainless steel fillers (under £200.00).] See recommendations on [https://www.facebook.com/groups/MilkTheFunk/permalink/1839193092775563/?comment_id=1839195342775338&comment_tracking=%7B%22tn%22%3A%22R9%22%7D this MTF thread].
 +
* [https://www.polsinelli.it/en/stainless-steel-bottle-filling-machine-P205.htm (UK) Polsinelli Stainless steel bottle filling machine; 3 spouts.]  See feedback on this filler in [https://www.facebook.com/groups/MilkTheFunk/permalink/2047515865276617/ this MTF thread].
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/2765591796802350/?comment_id=2765749193453277&reply_comment_id=2766396953388501&comment_tracking=%7B%22tn%22%3A%22R%22%7D Brad Erickson's DIY 4 head counter pressure filler posted on MTF.]
 +
 
 +
====Mid Tier Products====
 +
* [https://morewinepro.com/products/xpressfill-wine-bottle-filler-shelf-4-spout.html# Xpressfill wine bottle filler] and [https://morewinemaking.com/products/wine-bottle-filler-professional-6-spout-cart-pump-float-switch.html Wine Bottle Filler (Professional) - 6 Spout].  See [https://www.facebook.com/groups/MilkTheFunk/permalink/1395299310498279/?comment_id=1395486907146186&reply_comment_id=1395529840475226&comment_tracking=%7B%22tn%22%3A%22R0%22%7D Tim Vandergrift's and Levi's product feedback].
 +
* [http://www.gwkent.com/6-spout-gravity-filling-machine.html GW Kent 6 head filler (non-carbonated).]
 +
** [https://www.criveller.com/products/brewing/bottling-solutions/manual-bottling-systems/fillers/ Crivellar reseller.]
 +
** [https://www.facebook.com/groups/MilkTheFunk/permalink/1790615954299944/ MTF member reviews and recommendations on this product.]
 +
** [https://www.facebook.com/groups/MilkTheFunk/permalink/2622012581160273/ Tips on cleaning.]
 +
* [https://www.criveller.com/products/brewing/bottling-solutions/manual-bottling-systems/ Crivellar bottle fillers.]
 +
* [http://www.gwkent.com/4-spout-sparkling-filling-machine.html 4 Spout Sparkling Machine (can bottle uncarbed or carbed liquid)] <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1324183294276548/?comment_id=1414105878617622&comment_tracking=%7B%22tn%22%3A%22R0%22%7D  Conversation with Chris Anderson on MTF.  9/22/2016.]</ref>.
 +
* [http://easyfillermanufacturing.com/ Easy Filler Manufacturing 4 head fillers.]  See recommendations on this [https://www.facebook.com/groups/MilkTheFunk/permalink/1839233396104866/?comment_id=1839367476091458&comment_tracking=%7B%22tn%22%3A%22R%22%7D MTF thread].
 +
* [http://www.enotecnicapillan.it/index.php?option=com_zoo&view=item&layout=item&Itemid=370&lang=en (Italy/UK) Fillers Bacco, 2 and 4 head stainless steel.]
 +
* [https://www.facebook.com/dkadvancedtechnologies/videos/vb.932405850145927/1522136591172847/?type=2&theater DK Advanced Technologies 2 head corker/2 head capper for semi-automated cork and capping.]
 +
* [http://www.thevintnervault.com/product/4211/Bottle-Filler-Pro%2C-6-Spout-.html Vintners Vault 6 Spout Bottle Filler with Electric Float and Pump.]
 +
* [https://www.tenco.it/en/enolmaster-4-head-vacuum-filler Enolmaster 4 Head Vacuum Filler].  See [https://www.facebook.com/groups/MilkTheFunk/permalink/2274395339255334/ mixed reviews on MTF].
 +
* [https://www.levitatefab.com/shop-1 Levitate Fabrication 4 spout filler], see also [https://www.facebook.com/groups/MilkTheFunk/permalink/1839233396104866/?comment_id=1840187429342796&reply_comment_id=1840292722665600&comment_tracking=%7B%22tn%22%3A%22R%22%7D this MTF thread] on using this product with uncarbonated liquids.
 +
* [https://www.tcwequipment.com/products/mori-filler TCW Equipment Mori Filler; 4 or 6 spout.]
 +
* [http://www.microfillingsystems.com/ Micro Filling Systems bottler filler (counter pressure, but can be used without counter pressure).]
 +
* [https://www.kinnek.com/product/prospero-equipment-corp-gai-2004/ GAI 2004 at Kinnek.] See also [https://www.vigoltd.com/Catalogue/Fillers/4-head-stainless-steel-linear-syphon-filler Vigo Ltd.].
 +
 
 +
===Bottling Tanks===
 +
* [https://www.facebook.com/groups/MilkTheFunk/posts/7505316402829842 MTF thread on mixing/bottling tanks.]
 +
 
 +
===Wire Storage Containers===
 +
* [https://www.google.com/search?q=Collapsible+Wire+Container&ie=utf-8&oe=utf-8 Wire Storage Containers.]
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/1601974536497421/ MTF thread on using dividers/support beams to stack bottles in wire containers by Ryan Fields.]
 +
 
 +
===Label Makers===
 +
Efficient label makers starting at around $1500 USD.
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/1726210094073864/ MTF Facebook thread on label makers for small breweries.]
 +
 
 +
===Kegs===
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/1789433527751520/ MTF thread on one-way kegs for commercial brewers, including brand recommendations.]
 +
 
 +
===Density Meters===
 +
* [https://www.anton-paar.com/us-en/products/details/density-and-concentration-meter-easydens/ Anton Paar Easydens.]  See also this [https://www.facebook.com/groups/MilkTheFunk/permalink/1877369832291222/ MTF thread for reviews on this product].
 +
* [https://www.facebook.com/groups/MilkTheFunk/permalink/3381707858524071/ MTF thread on a simple CO<sub>2</sub> gauge that uses a swing top bottle to measure CO<sub>2</sub> during bottle fermentation.]
 +
 
 +
===Cappers===
 +
* [https://www.amazon.com/North-Mountain-Supply/dp/B0CGVVLSPD?th=1 Italian-made Heavy Duty Bench Capper ($100).]
 +
* [https://morebeerpro.com/products/deluxe-bench-capper.html Deluxe Bench Capper ($135).]
 +
* [https://morebeerpro.com/products/ferrari-pneumatic-bottle-capper.html Ferrari Pneumatic Bottle Capper ($680).]
 +
* [https://www.gwkent.com/pneumatic-capper-air-matic.html GW Kent Pneumatic Capper Air-Matic ($945).]
 +
* [https://www.elnova.ca/en/equipment/bottling/cappers-crimpers/897-pneumatic-capper.html Pneumatic capper (Quebec, Canada).]
 +
 
 +
===Misc===
 +
* [https://www.morebeer.com/products/bottle-rinsing-purging-base.html MoreBeer Bottle Washer and Purger.] <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/2070646812963522/?comment_id=2071602452867958&comment_tracking=%7B%22tn%22%3A%22R0%22%7D Rowan Chadwick.  Milk The Funk Facebook group thread on bottle cleaners for small brewers.  04/25/2018.]</ref>
 +
 
 +
==Mobile Canners==
 +
Companies that can sour/funky beer.
 +
* [https://www.facebook.com/icanbeer/ ICan Solutions, LLC from Indiana.]
  
 
==Storage==
 
==Storage==
Line 77: Line 344:
  
 
===Additional Articles on MTF Wiki===
 
===Additional Articles on MTF Wiki===
 +
* [[Floor Corker]]
 +
* [[Corking]]
 
* [[Blending]]
 
* [[Blending]]
 
* [[Aging and Storage]]
 
* [[Aging and Storage]]
 
* [[Tetrahydropyridine]]
 
* [[Tetrahydropyridine]]
* [[Floor Corker]]
 
 
* [[Mixed Fermentation]]
 
* [[Mixed Fermentation]]
 
* [[Funky Mixed Fermentations]]
 
* [[Funky Mixed Fermentations]]
Line 86: Line 354:
  
 
===External Resources===  
 
===External Resources===  
 +
* [https://topcrop.co/bottle-keg-conditioning-an-introduction "Bottle and Keg Conditioning, an Introduction" by Top Crop (Omega Labs; Nik Allen and Adi Hastings).]
 
* [http://jeffreycrane.blogspot.com/2015/06/blending-calculator-ph-abv-and.html "Blending and Priming Calculator," by Jeff Crane.]
 
* [http://jeffreycrane.blogspot.com/2015/06/blending-calculator-ph-abv-and.html "Blending and Priming Calculator," by Jeff Crane.]
 
* [http://sourbeerblog.com/carbonation-in-sour-beers/ "Carbonation in Sour Beers," Sour Beer Blog.]
 
* [http://sourbeerblog.com/carbonation-in-sour-beers/ "Carbonation in Sour Beers," Sour Beer Blog.]
 
* [http://www.themadfermentationist.com/2009/11/brewing-sour-beer-at-home.html "Brewing Sour Beer At Home," by Michael Tonsmeire (see the "Bottling" section).]
 
* [http://www.themadfermentationist.com/2009/11/brewing-sour-beer-at-home.html "Brewing Sour Beer At Home," by Michael Tonsmeire (see the "Bottling" section).]
 
* [http://kotmf.com/tools/prime.php Priming calculator that includes entering a value for residual CO2.]
 
* [http://kotmf.com/tools/prime.php Priming calculator that includes entering a value for residual CO2.]
* [https://www.facebook.com/groups/MilkTheFunk/permalink/1244463818915163/ MTF Thread on 4-6 spout gravity-fed bottling lines.]
+
* [https://tapintohach.com/ Tap Into Hach; blog about understanding the technical aspects of and dealing with dissolved oxygen in beer.]
* [https://www.homebrewersassociation.org/wp-content/uploads/MJzym06_Champagne.pdf "Et Tu, Brut: Brewing the Champagne of Beers," by Drew Beechum.]
 
  
 
==References==
 
==References==

Latest revision as of 20:03, 29 October 2024

Packaging is the transfer of your mixed fermentation/sour/funky beer from a fermentation or aging vessel into the the final package (e.g. a bottle or a keg). This page will discuss various things one should consider such as when to package, how to package and what final package to put the beer into. For information on packaging with corks and in large format bottles, see the Corking page. See Aging and Storage for storage information.

When to package

Final Gravity

There is no hard and fast rule for when to package based on final gravity because the gravity of the finished beer will depend on recipe, process, and microbes present among other factors. Generally, lower final gravities are safer because they constrain the extent to which the beer can continue to ferment and generate extra CO2 in the package. But rather than looking for a specific number, pay more attention to the stability of the gravity. Jay from the Rare Barrel recommends waiting at the final gravity for 2-3 months in mixed fermentation beers to ensure that this gravity is the actual final gravity [1] (~38 minutes in). If the gravity is stable over a long time scale (weeks to months, depending again on factors such as the recipe, process, and microbes present), then the beer is probably done. If you are unsure then give the beer a bit longer and monitor final gravity. Keep in mind that sampling sour beer too often can lead to too much oxygen exposure, and care should be taken not not sample too much and purge with CO2; see Mixed Fermentation for more information. Each gravity point (.001) of continued fermentation yields roughly 1/2 volume of CO2 (each degree Plato yields 2 volumes of CO2) [2]. Sometimes beer samples will contain high amounts of carbonation due to gradual fermentation from Brettanomyces (in other cases when the beer has aged for a very long time, there may be much less residual carbonation than normal). Therefore, it is best practice to degas the sample by pouring it through a membrane filter such as a coffee filter in order to obtain an accurate gravity reading [3].

With continual batches of beer using the same grist and strains of microbes, a stable final gravity can become more easily predicted by the brewer and waiting for a stable final gravity may no longer be necessary once a known stable final gravity is achieved (note that a change in the strains being used or the grist could result in a different stable final gravity). For example, see the Brettanomyces and Saccharomyces Co-fermentation page for techniques for inoculating Brettanomyces at bottling time into a clean beer that was fermented with just S. cerevisiae.

If fruit was added to an already matured mixed fermentation beer with living Brettanomyces in it, an additional 1-2 months should be given after adding the fruit to ensure that the sugars from the fruit are fully fermented. See Soured Fruit Beer Refermentation for details on using fruit in sour beers.

For suggested equipment for reading gravity from small samples, see Density Meters.

Flavor Development

While determining if the final gravity is stable is the most important factor when choosing when to package mixed fermentation beer, there are also different approaches to choosing when to package beer based on the flavor maturity of the beer. For Mixed Fermentation beers that contain Brettanomyces and lactic acid bacteria, months are often required for the beer to reach an optimal or even just acceptable flavor profile. This depends on many variables such as is the beer aging in an oak Barrel, what microbes are pitched (for example, Pediococcus and slower species of Lactobacillus such as L. delbruekii tend to need a few months to develop acidity), what flavors the brewer is looking for, etc. If the gravity is stable, some brewers prefer the option of packaging the beer before the flavor is optimal and allowing the flavor to continue to develop in the bottle/keg. This gives the advantage of being able to sample the beer at different points in time and can serve as a good technique for learning how a particular culture ages. If this approach is taken, keeping the bottles/kegs at room temperature instead of at refrigerator or cellar temperatures is advised so that the yeast and bacteria do not fall dormant. Colder temperatures will slow the maturation of the beer. Other brewers prefer to allow the maturation of flavors to happen in the fermenting vessel (especially in the case of oak barrels), and then package the beer when it tastes good. This approach is less risky as far as potentially packaging a premature beer that never fully reaches the flavor profile that the brewer finds acceptable. In other words, sometimes mixed fermentation beers never taste good, and allowing the beer to bulk age in the fermenting vessel allows the brewer to make sure the beer is worth packaging.

For more information on when mixed fermentation beers are ready to package based on flavor, see the various Brewing Techniques pages. For more information on aging and cellaring packaged beer, see Aging and Storage.

Clarity and pellicles

Beer with Brettanomyces and bacteria, as well as certain Saccharomyces strains, will take longer to clear than other beers. Generally, time can take care of most of the clarification in mixed fermentation beers with long aging times. Faster turnaround beers or kettle sours may not clarify by time alone. The level of clarity you are looking for before packaging will depend on your preferences and the beer you are making. Beer can be packaged without good clarity and they will continue to settle out in the bottle, but note that beers with high carbonation levels may kick the sediment back into suspension, which may or may not be what you want in your beer.

Some producers will coarse filter their fruited or dry hopped sour beers to prevent pieces of fruit from clogging equipment or making it to the package [4][5]

Beer with a pellicle can be bottled, and it is not necessary to wait for a pellicle to disappear before bottling. See Pellicle for more information.

How to package

General

Re-yeasting

When bottling or priming a keg at packaging time, the brewer should consider re-yeasting. In a long aged beer, especially an acidic beer, the Saccharomyces will mostly be dead already. This leaves Brettanomyces in charge of conditioning the beer. Although some lactic acid bacteria are capable of producing CO2, their contribution is probably negligible. It is also possible that homofermentative LAB will consume a portion of the sugar before the Brett has a chance to produce CO2. Re-yeasting is a very effective way to ensure proper CO2 levels in an aged sour beer. Another benefit of re-yeasting is that it tends to help avoid (or minimize) Tetrahydropyridine production.

Commercial producers and MTFers have had success re-yeasting with their mixed culture, wine yeast, and champagne yeast. The specific yeast you choose is up to you, and we recommend that you try a couple different yeasts out to find the one you prefer (MTFers have reported success with Lalvin EC-1118 Champagne, Red Star Premier Cuvée, Red Star Pasteur Blanc, Danstar CBC-1, Fermentis T-58, Scott Laboratories DV10 wine yeast, and fresh cultures of Brettanomyces [6]). When re-yeasting at bottling for a beer that has some residual gravity (a higher than 0 plato or specific gravity), take care not to use a highly attenuative yeast that could over-attenuate the beer in the package. For example, adding Brettanomyces or a diastatic strain of Saccharomyces cerevisiae at bottling to a beer that has some residual sugars could result in over-carbonation in the bottle [1] (~41 minutes in). If the beer has a significantly high finishing gravity, then re-yeasting with a yeast that beer has already seen should eliminate the possibility of continued attenuation as long as the beer is already at final gravity. Using rehydrated dry yeast has been shown to be more reliable than both slurries and even freshly propagated yeast probably due to their rich sterol reserves (this excludes freshly propagated yeast using acid shock starters - see below) [7][8]. Brewers who are having difficulty carbonating sour beer or need to ensure that the beer will carbonate properly should grow their yeast first in an Acid Shock Starter. This will acclimate the yeast to the harsh conditions of the sour beer, and has shown to be more effective for ensuring bottle carbonation [9]. Not all sour beers will need the priming yeast to be acclimated, but sour beers with darker malts, acidic fruits, and high ABV (8%+) might require this step. See Acid Shock Starters below, and fermenting in low pH conditions for more information on the science behind this technique.

The yeast required for carbonation is very little. A good rule of thumb to use is to use 10% of the yeast that you would normally use for a primary fermentation (approximately 1 million cells per mL). For example, for dried yeast use ~2 grams of yeast for 5 gallons of beer [10]. Rehydrating the yeast is recommended. See Jeff Crane's "Blending Calculator" (extension of Michael Tonsmeire's "Blending Calculator") for a re-yeasting and priming calculator. Dry yeast should be rehydrated in ten times its weight in 80-90°F (27-32°C) water for ~15-20 minutes [11].

Many wine yeast strains are known to be "killer" yeast strains. In Saccharomyces, killer strains produce toxins that kill sensitive strains. Neutral strains do not produce toxins, nor are they killed by them [12]. Almost all ale and lager strains are sensitive to the toxins produced by killer strains [13][14]. In Saccharomyces, four toxins have been identified: K1, K2, K28, and Klus, the first three of which can only kill other strains/species of Saccharomyces. The Klus toxin has been found to kill yeast from other genra, such as Hanseniaspora spp., Kluyveromyces lactis, Candida albicans, Candida dubliniensis, Candida kefir and Candida tropicalis, and the K1, K2 and K28 killer strains of S. cerevisiae [15]. However, none of the toxins have been found to kill Brettanomyces [16]. The K1 toxin is most active between a pH of 4.6 and 4.8, while K2 and Klus are active around a pH of 4.0 to 4.3 [15]. See Killer Wine Yeast for more information on these toxins.

This tends to create some fears for brewers who believe that the Saccharomyces strains in their beer might still be alive. However, in a highly acidic sour beer, Saccharomyces wine strains tend not to live for extended periods of time [17]. Even if some cells do, their activity would be next to none due to no available sugars left in the beer (other than priming sugar), and thus their contribution to the flavor development of the beer would be minimal to none. Assuming good bottling practices are followed, fear of autolysis occurring due to killer strain activity is usually unfounded because most of the Saccharomyces cells are left behind with the trub at bottling. Additionally, Brettanomyces has the ability to metabolize the acids and proteins that are released during whatever autolysis that might occur in the bottle. Re-yeasting with wine yeast for priming has the additional advantage of not fermenting maltose or maltotriose, so unexpected attenuation from the wine yeast will generally not occur as long as the beer is already fully attenuated. Thus, there is little argument against re-yeasting with wine yeast at packaging time, other than a desire to approach carbonation in a traditional sense such as is the case with lambic.

The flavor impact by the wine yeast in beers that contain living Brettanomyces is also probably minimal or not significant, although this has yet to be established scientifically [18]. This would be due to the small amount of fermentables provided by the priming sugar, as well as the ability of Brettanomyces to metabolize many secondary metabolites that would be produced by a bottling yeast. It has been shown, however, that bottling yeast in general can have a flavor impact on clean beers without Brettanomyces. For example one study found that out of 5 dried yeast strains from the Lallemand Yeast Culture Collection (Montreal, QC, Canada), one strain produced a higher final pH by about ~.05 (from 3.98 to 4.02 pH) in a Belgian dark ale (no Brettanomyces or other non-traditional microbes were used in the beer). The various strains tested also affected the levels of acetic acid, ethyl acetate, isoamyl alcohol, acetaldehyde, diacetyl, and glycerol in beer just from bottle conditioning [11]. Some brewers speculate that thiols from wine yeast could also create novel characteristics in beer (although perhaps not from only bottle conditioning yeast) [19]. It could therefore be possible that metabolites produced from bottling yeast could have an effect on sour/"Brett" beer flavor as well, but this remains to be demonstrated scientifically.

Acid Shock Starters

Below are several acid shock starter techniques for acclimating yeast to the harsh conditions of acidic wort or beer, including mixed fermentation sour beers and kettle sour beers. See fermenting Saccharomyces under low pH conditions for more information on the science behind this.

Escarpment Labs Acclimation Protocol

The following acclimation protocol has been adapted for sour beer from Richard Preiss's instructions on how Escarpment Labs acclimates wine yeast: [20]

  1. Take a "pinch" of rehydrated dried yeast, and pitch 150,000 cells/mL into 100 mL of YPD + Go-Ferm Media. Grow for ~24 hours at 86°F (30°C) with shaking.
  2. Transfer the starter into 100 mL of Adaption Media 1. Grow for ~24 hours at 86°F (30°C) without shaking.
  3. Transfer the starter into Adaption Media 2 (whatever volume is needed for batch size). Grow for ~24 hours at 86°F (30°C) without shaking.
  4. Pitch ~1 million cells per mL of sour beer that is being carbonated, along with priming sugar.

Definitions:

YPD + Go-Ferm Media (autoclaved)
0.02375 g/L Go-Ferm
10 g/L yeast extract
20 g/L peptone
20 g/L dextrose
Adaption Media 1 (filter-sterilized)
10% base sour beer
20 g/L sucrose
0.02375 g/L Go-Ferm
Adaption Media 2 (filter-sterilized)
70% base sour beer
20 g/L sucrose
0.2 g/L Fermaid

Homebrew/Low Tech Acclimation Method

This method was adapted from the Escarpment Labs and the Rogers et al. methods using non-laboratory processes and ingredients.

This method has not yet been validated as far as producing the correct pitching rate for re-yeasting, which is suggested to be 1 million cells per mL of beer.

For ~20 liters (or around 5-6 gallons) of sour beer:

  1. Measure 1-2 grams of dried yeast, and re-hydrate in a little bit of warm water.
  2. Measure 50 mL of the sour beer that is going to be carbonated, and combine it with 100 mL of apple juice with no preservatives (or use DME if using a beer yeast strain) and a pinch of Go-Ferm in a saucepan.
  3. Heat pasteurize the sour beer, apple juice, and Go-Ferm mixture on the stove at 160°F for 10 minutes.
  4. Cool the sour beer and apple juice mixture to around 86°F (30°C). Pitch into an Erlenmeyer flask, along with the re-hydrated wine yeast.
  5. Grow for ~24 hours without shaking at around 86°F (30°C) *.
  6. Measure 200 mL of sour beer that is going to be carbonated, and combine with 200 mL of apple juice and a pinch of Go-Ferm in a saucepan.
  7. Heat pasteurize the sour beer, apple juice (make sure there are no preservatives in the apple juice), and Go-Ferm mixture on the stove at 160°F for 10 minutes.
  8. Cool the sour beer and apple juice mixture to around 86°F (30°C). Top up the Erlenmeyer flask with the yeast starter in it with this fresh mixture.
  9. Grow for ~24 hours without shaking at around 86°F (30°C) *.
  10. Continue to step up the starter as needed for larger batches.
  11. After the 24 hours of growth is finished for the final step, add ~200 mL of the starter per 20 liters of beer (stir the starter before adding into the beer; this does not account for the sugar that needs to be added to achieve carbonation because the apple juice should be fully fermented at this stage).

* If a fermentation temperature of around 86°F (30°C) cannot be maintained and the temperature falls during the 24 hour growth period, allow the final step to ferment for 48 hours before adding it to the beer just to make sure the apple juice is fully fermented.

Priming

If your beer is at final gravity, you can prime it as you would any normal beer based on the amount carbonation you want. In order to properly prime your beer, you will need to know your residual CO2 in solution. This can be determined by temperature and good priming sugar calculators take this into account. For aged beers, especially beers which have been aged in barrels or a similar porous vessel, the starting CO2 may be lower than the numbers produced from these calculators due to off-gassing while the beer is aging. We recommend using Jeff Crane's "Blending Calculator" (extension of Michael Tonsmeire's "Blending Calculator") for priming calculations with aged beer.

Tomme Arthur from The Lost Abbey reports that the starting values he sees in barrel aged beers range from 0.2-0.6 volumes of CO2[21] (~51 minutes in). Brewers may choose to partially force carbonate their beers to know the starting CO2 exactly as well as limit the CO2 required from bottle conditioning. The Lost Abbey carbonates to 1.5 volumes of CO2 before priming and bottle conditioning for the remaining carbonation[21] (~52 minutes in). Alternatively, a product used in measuring residual CO2 for champagne called a Carbodoseur can be used to estimate residual CO2 after long aging. See these MTF threads on experiences using the Carbodoseur and the Carbodoseur calibration table. If no effort is made to determine residual CO2, 0.4 has been quoted as a good estimation for aged beer [22].

If your beer is not at final gravity, but you have a good idea of where it will finish, then you can prime taking into account remaining gravity points. See Final Gravity for more information.

Alternatively, beer can be kegged and force carbonated like any other beer. Beer can be then bottle/crowler/can filled from the keg using a beer gun or some other device. Anecdotes from some brewers have reported a higher chance of THP production when force carbonating some mixed fermentation beers (see the THP page for more information). See Package Types below for more information on kegging in general.

CO2 levels in bottles can be measured with special equipment. For example, Allagash uses a Haffmans CO2-Selector. The pierce model Zahm can also be used to measure packaged CO2 levels (see this MTF thread).

See also:

Bob Sylvester's Priming Guide

Bob Sylvester from Brasserie Saint Somewhere describes his method to bottle conditioning saison/farmhouse style beers on a commercial scale (amounts are for a 7 barrel/217 gallon batch of beer) [23]. This method achieves high carbonation levels that are appropriate for saison styles, which targets about 2.5 volumes and is low enough to account for some minor attenuation in the bottle [24]. The sugar levels listed assume the beer is not barrel aged and has residual volumes of CO2 (approximately 0.86) [25]:

  1. All my beers have a final gravity of pretty much zero. I use plain cane sugar. I've found it to be pretty yeast friendly and the results are faster with sucrose. Not sure why dextrose became popular. Most of us don't brew with corn, so why add it at packaging.
  2. Bob prefers Red Star brand dry champagne yeast. Suggested alternatives include EDM wine yeast, Maurivin PDM, and Fermentis Safoeno VR 44 [26].
  3. For 7 barrels/217 gallons of beer, dissolve the 12 pounds of cane sugar (0.88 ounces of sugar per 1 gallon of beer) in ~3.5 gallons of 108-110°F water. After the sugar dissolves, add 250 grams of yeast to the sugar water (1.15 grams of yeast and 0.885 oz of sucrose dissolved in 2 fl oz of water per gallon of beer, or 6.628 grams of sucrose per liter of beer). The amount of yeast is less important than the amount of sugar [26])[27]. I do this first and let it bloom while I transfer the beer from the fermenter to my kettle. Takes about 20-30 minutes. By this time the yeast is about to crawl out of the bucket. Editor's note: many homebrewers have had success carbonating sour beer with only 1-2 grams of wine yeast for 5 gallons of beer.
  4. Add the yeast/sugar slurry just before the transfer is finished as this will help incorporate the slurry. Carbonation happens the same day as packaging if done correctly.
  5. Sounds counter to everything you've learned, but don't gas purge the bottles. The yeast will uptake the O2 in the head space.
  6. Sanke keg conditioning uses the same exact process but back the sugar down to 8 pounds (0.59 ounces of sucrose per gallon of beer or 4.4186 grams of sucrose per liter of beer) and keep the yeast at 250 grams. Fill the keg to the shoulder where the side and the lid meet (too much headspace leads to too high of a carbonation level). One recommendation for getting the right headspace is to lift one side of the keg onto a 2x4 wood block and fill it while the keg is leaning at an angle (this method works best for half barrel kegs; smaller kegs might tip over) [28].
    1. Note: For a discussion on why or why not the dosage of priming sugar for kegs should be lower than for bottles, see this thread.
  7. Wait 10 days. That's it. With time, the initial "rough" carbonation you get in the first couple days will mellow out into the fluffy stuff.

Krausening

David Fuhrer reported using a "speise" method similar to krausening for mixed fermentation beers reduced the time of THP in mixed fermentation bottles to 3 weeks. See Episode 237 of the MBAA podcast and related links.

See also:

Oxygen exposure

Oxygen is the enemy of finished beer, and most producers of standard beers invest a lot of time and money into reducing oxygen exposure in their finished beer (referred to as "dissolved oxygen" for oxygen in the beer or "total packaged oxygen" for total oxygen in the beer plus additional oxygen in the headspace of the package). Excessive oxygen exposure can negatively impact the beer either permanently through the formation of acetic acid and/or ethyl acetate, temporarily by increasing tetrahydropyridine (THP) formation, or through the oxidation of various compounds. For mixed fermentation beers where fresh bottling yeast is added, this may not be as significant of a concern, depending on how much oxygen is allowed to enter the packaging. These beers, especially barrel aged versions, generally have more oxygen exposure during fermentation and aging, and this contributes to the characteristics of the beer. Brettanomyces is known as an "oxygen scavenger", which helps to prevent oxidative reactions in the bottle over time (see Aging and Storage). Fresh bottle conditioning yeast can help prevent acetic acid and THP production by consuming the oxygen in non-purged bottles before it can have an effect on the mixed culture. Even without fresh bottling yeast, some brewers achieve good results without purging bottles before filling (for example, lambic producers and blenders), although this might be dependent on whether the strains present in the beer are big THP producers or if the brewer has limited time to store bottles long enough for the THP to age out. Others do take care to avoid oxygen exposure in the packaging process and report that purging bottles with CO2 helps prevent THP formation. Due to these many variables, there is no easy answer in terms of whether it is better to carefully CO2 purge as is standard practice for clean beers. CO2 purging can require more expensive equipment, as well as require more time to package. Based on the characteristics of the microbes being used, experience, equipment, process, and preferences, the brewer can determine how much limiting O2 exposure during packaging is necessary. The safest route is to treat them as you would a normal beer and take care to avoid O2 where this is possible or affordable, though the experience of some will say that this extra work and cost might not be completely necessary like it is for clean beers.

If force carbonating the beer instead of bottle conditioning with fresh yeast, or if bottle conditioning without adding fresh yeast, it might be best practice to purge bottles and kegs with CO2 in order to limit the production of both acetic acid and THP. Again, experience should drive this decision one way or the other.

See also:

MBAA podcasts:

Package and closure types

(in progress) Many sour/funky/mixed fermentation beers are highly carbonated. It is recommended to package highly carbonated beers in bottles of thicker glass which can better withstand higher pressure (e.g. many Belgian and German bottles, corkable Champagne-type or 'fat-lipped' Belgian-type bottles, etc.). This is especially true if you feel the beer may continue to carbonate in the bottle from residual carbohydrates beyond the priming sugar you added. Be careful when carbonating your beer to high volumes to ensure that your chosen package can handle this pressure.

For information about corking, see the Corking page.

  • Crown caps (26 mm) - A variety of thicker glass/high-pressure bottles are available in the standard 26 mm cap size. No special equipment, beyond a normal capper, is necessary to fill bottles of this type. It may be necessary to swap the metal plates on cappers such as the Emily capper to accomodate the wider neck on some bottles taking a 26 mm cap (such as champagne-style bottles with 26 mm caps (e.g. Logsdon, Goose Island, some Upright) and the 375 mL crown finish bottles modeled after the 'Vinnie' 375 mL bottles).
  • Crown caps (29 mm) - For capping bottles with 29 mm openings (such as Champagne-style bottles and 375 mL half-Champagne bottles), you will need to source a 29 mm bell and 29 mm caps, which are non-standard. Many 29 mm bottles are both capable and corkable. For cork and capping, see below.
  • Swing tops - Many swing tops are thicker glass and are therefore well suited for higher carbonation beers. Swing tops also allow for easy venting of carbonation if the beer carbonates more than desired/anticipated. Due to the soft gasket (and possibly plastic top piece holding the gasket for some bottles), the same concerns regarding cleaning difficulty and cross-contamination apply to swing tops as to other plastic parts used after the hot side when brewing both normal and 'funky' beers. If you don't want your normal beers exposed to the micro-organisms in your funky beers, we recommend not using the same swing tops for bottling both types of beer, jusrt as we recommend keeping seperate sets of plastic equipment used for fermentation and transfer of fermented beer when brewing both clean and 'funky' beers. Some express concern about long term aging in swing tops and the possibility of swing tops not being as impermeable a barrier to O2. If you are concerned about this they it may be better to not use swing tops for long aged beer. At this time we are unaware of any comparisons of swing tops and other closure mechanisms regarding their susceptibility to O2 transfer during extended aging to give a firm answer as to whether swing tops allow more O2 ingress than other closures or not .
  • Kegging - Kegging offers the benefit of being able to force carbonate or naturally carbonate with adding priming sugar and (optionally) re-yeasting. If force carbonating, follow the same procedures that you would for any other beer. Consider the "set and forget" method of force carbonating, which requires 1-3 weeks. This will allow the beer to settle while also dialing in the carbonation level. Priming in the keg often produces great results with sour beers. Generally, use 1/2 of the priming sugar that you would normally use, or excessive foaming can occur [29]. If re-yeasting, which is generally recommended (see the Re-yeasting section above), use 10% of the yeast you would normally use to for a primary fermentation. For example, use around 2 grams of dried champagne yeast re-hydrated to naturally carbonate 5 gallons of beer [10]. Priming in the keg will result in a little more sediment at the bottom of the keg, but this will be pulled out on the first pour from the keg. Allow at least three weeks of conditioning at room temperature, and then a week at refrigeration temperatures before serving. If the keg is not re-yeasted, it might take considerably longer than three weeks to carbonate the beer depending on the vitality of the yeast in the beer. The brewer should consider keeping plastic kegging equipment such as serving lines and taps separate from kegs that serve clean beers.
A spunding valve can also be used to help guard against over-carbonation when keg conditioning. The CO2 gauges on spunding valves can be inaccurate, however, so finding the correct PSI setting requires the following technique. The goal is to set it to be around 30 PSI for room temperature conditioning. First, pressurize the keg to 30 PSI using a reliable CO2 regulator, then remove the gas line while the keg is still pressurized. Fully tighten the spunding valve's pressure release valve (PRV) so that no gas is released, and attach it to the keg's gas line. Slowly turn the PRV on the spunding valve so that the gas is released. Continue to release the gas until the faintest "hiss" of gas can be heard, and then slightly tighten the PRV. The PRV might be set to something like 40 or 50 PSI, but the reliable CO2 regulator can be used to verify that the spunding valve's PRV is set to the desired level. The actual PSI within the keg might oscillate around 27-32 PSI, so this technique might need some trial and error. Shoot for under-carbonation versus over-carbonation. If the beer is slightly under-carbonated, force carbonation can make up the difference. Slight over-carbonation should be avoided because it could make the beer very difficult to serve [30].
Another method for setting the spunding valve's PRV to the correct PSI setting would be to use a keg of beer that is already perfectly carbonated. Allow the keg to warm to conditioning temperatures. Attach the spunding valve with the PRV completely closed. Slowly release the PRV until you hear a slight hiss. This should reach the PSI setting that the spunding valve needs to be set at to reach around the same carbonation for other kegs. Kegs should be filled up to the same headspace as the keg that was used to set the PRV setting [31].
  • Green glass v Brown glass - Some bottle types, especially champagne style bottles, may be more available in green glass compared to brown glass. Green glass does a worse job shielding the beer from UV spectrum light, which can interact with hop compounds to produce 'skunky' or lightstruck flavors and aromas. Many classic Belgian mixed fermentation beers are found in green glass, and some producers in North America seek out green glass over brown glass for their beers [32][33] (~11 minutes in) (also, Bob Sylvester). See also Lightstruck.
  • Crown caps v corks - Deciding between corks and caps will depend on the specific beer and the brewer's goals with the beer. Caps are a cheaper closure and are also easier to apply. Aside from aesthetics, you may choose corks when intending to age or store beers lying down in order to keep the beer from interacting with the cap. Some producers have observed that crown caps contribute to an off-flavor in their beer, especially if the beer is acidic. Pierre Tilquin closes some bottles of every blend with crown caps in order to test carbonation levels. He has noticed that the crown capped bottles have an off flavor not present in the corked bottles [34]. Other producers regularly package their acidic beer with crown caps (e.g. the Rare Barrel, Boon Geuze in 25 cl bottles) and have not mentioned a problem with doing so.

Corking

See Corking and Floor Corker fore more information on corking and using floor corkers.

Canning

Usually, only pasteurized sour beer is canned due to the danger of over-carbonating canned beer, however, some brewers have been experimenting with canning mixed fermentation beer and allowing the beer to naturally carbonate in the cans. It is recommended that brewers contact their can supplier to verify that the plastic liners for the cans are rated to handle the Titratable Acidity of sour beer (kettle soured or otherwise) and to find out the lifespan of the cans. Cans have been found to preserve some hop compounds such as myrcene and caryophyllene better than bottles because bottle caps can strip some of these compounds [35].

One study by Sheehan et al. (2024) examined 9 different sour beers canned with 4 different liners with their maximum thickness available from the manufacturer: BPA based epoxy, acrylic, and two versions of BPA-NI epoxies. They aged the cans up to 48 weeks at 20°C. Out of the 9 different sour beers examined, they found that one sour beer with 7.55 g/L lactic acid (the other sour beers that did not heavily erode the liners had 5.84 g/L or less lactic acid) and one sour beer with 9.01 g/L acetic acid eroded the liners and increased the dissolved aluminum to elevated levels of 9.3 mg/L and 43.2 mg/L. This occurred at the 48 week mark. The European Food Safety Authority advises consumption of no more than 1 mg/kg body weight per week; the World Health Organization advises a limit of 2 mg/kg of body weight per week. Aluminum is regularly used as a food additive in small amounts, and most aluminum consumed is filtered by the kidneys. Regular exceedence of the weekly intake can increase the risk of health impairments, including developmental toxicity, neurotoxicity, and effects on the urinary tract and potentially the digestive track. There is no consistent evidence linking aluminum exposure from food and water to Alzheimer's disease or breast cancer. All 4 liner types were eroded by the two beers and did not comparatively protect the beers from aluminum contamination. For the beer with high acetic acid, which was deemed not sellable by the brewery that produced it and dumped, consumption of a 355 mL (12 oz) serving per day would equate to ~15 mg/day aluminum, or ~100 mg/week. This value approaches the World Health Organization recommended total weekly intake of <2 mg Al/week per kg body weight, or <150 mg Al/week for a 75 kg person. Can erosion was not correlated with pH, but it was correlated with Titratable Acidity. It is recommended that breweries send samples of their beer to their can supplier to have the beer tested to make sure that the liner will not erode [36][37][38]. See also this post on MTF.

See also:

Capping

Using Sulfite

Sulfite is generally not added to mixed fermentation beer. It may be possible to use sulfite to inhibit refermentation of unfermented sugars. There is also some speculation that sulfite might help reduce Tetrahydropyridine.

Equipment

Bottle Suppliers

Single Cases

Bulk

Bulk 29mm Caps

See also:

Corkers, Corks, and Cages

See Corking.

Bottle Fillers

Cheap and DIY

Mid Tier Products

Bottling Tanks

Wire Storage Containers

Label Makers

Efficient label makers starting at around $1500 USD.

Kegs

Density Meters

Cappers

Misc

Mobile Canners

Companies that can sour/funky beer.

Storage

See Also

Additional Articles on MTF Wiki

External Resources

References

  1. 1.0 1.1 The Sour Hour episode 6 Listener Q&A
  2. "Accurately Calculating Sugar Additions for Carbonation." Kai Troester. Braukaiser.com. Retrieved 08/07/2016.
  3. "Recommended Beer Degassing Methods and Alternatives Matrix". ASBC Methods of Analysis. Retrieved 03/04/2020.
  4. Casey Brewing & Blending fruit strainer
  5. Jean at Cantillon making a filter
  6. Conversation on MTF regarding bottle conditioning yeast. 11/21/2016.
  7. an Landschoot, A., Vanbeneden, N., Vanderputten, D. and Derdelinckx, G. (2004). Effect of pitching yeast preparation on the refermentation of beer in bottles. Cerevisia 29:140-147.
  8. Van den Berg, S., Demeyere, K. and Van Landschoot, A. (2001). The use of dried yeast for the bottle refermentation of beer. Cerevisia 26:102-108.
  9. Terminal acidic shock inhibits sour beer bottle conditioning by Saccharomyces cerevisiae. Cody M. Rogers, Devon Veatch, Adam Covey, Caleb Staton, Matthew L. Bochman. 2016.
  10. 10.0 10.1 Blending Calculator - pH, ABV and Carbonation. Jeff Crane. Blending Calculator - pH, ABV and Carbonation. Bikes, Beer, & Adventures Blog. June 12, 2015.
  11. 11.0 11.1 S. M. Van Zandycke, T. Fischborn, D. Peterson, G. Oliver, and C. D. Powell (2011). The Use of Dry Yeast for Bottle Conditioning. MBAA Technical Quarterly. doi:10.1094/TQ-48-1-0225-01.
  12. Advances in Microbial Physiology, Volume 22. Academic Press, Sep 15, 1981. Pg 94-95.
  13. Conversation with Bryan of Sui Generis Blog on MTF on Killer Factor for Saccharomyces. 11/16/2015.
  14. Strains of Yeast Lethal to Brewery Yeasts. A.P. Maule and P.D. Thomas. 1972.
  15. 15.0 15.1 A New Wine Saccharomyces cerevisiae Killer Toxin (Klus), Encoded by a Double-Stranded RNA Virus, with Broad Antifungal Activity Is Evolutionarily Related to a Chromosomal Host Gene. Nieves Rodríguez-Cousiño, Matilde Maqueda, Jesús Ambrona, Emiliano Zamora, Rosa Esteban and Manuel Ramírez. 2011
  16. Non-Saccharomyces killer toxins: Possible biocontrol agents against Brettanomyces in wine? S. Afr. J. Enol. Vitic. vol.36 n.1 Stellenbosch. 2015.
  17. Conversation with Richard Preiss on MTF. 03/28/2016.
  18. Conversation with Richard Preiss on MTF. 02/15/2017.
  19. Conversation with James Sites about thiol producing wine strains on MTF. 08/19/2016.
  20. Wine yeast acclimation protocol for Escarpment Labs by Richard Preiss. 08/01/2016.
  21. 21.0 21.1 Tomme Arthur from the Lost Abbey on the Sour Hour
  22. Tonsmeire, Michael. American Sour Beer: Innovative Techniques for Mixed Fermentations. Brewers Publications. 06/15/2014. ISBN-13:9781938469138. Pg 271.
  23. Conversation with Bob Sylvester on his bottle conditioning methods on MTF. 09/26/2015.
  24. Brewers Friend's priming calculator.
  25. Discussion on MTF with Bob Sylvester, clarifying residual CO2 levels in his finished beer. 11/07/2016.
  26. 26.0 26.1 "Kathleen Round 3 - More Hops!" The Farmhouse Obsession Blog by Andrew "Gus" Addkison. 06/08/2016. Retrieved 06/08/2016.
  27. Bob Sylvester on rehydrating yeast and sugar together. Milk The Funk Facebook group. 12/13/2017.
  28. Bob Sylvester and Allen Stone. Milk The Funk Facebook group thread about keg conditioning and fill level. 10/26/2018.
  29. Don Million. Keg Your Beers! Brew Your Own Magazine. 2003.
  30. Keg conditioning thread on MTF with Bryan from Sui Generis and Devin Henry. 08/12/2016.
  31. Keg conditioning thread on MTF with Taylor John Caron. 11/07/2016.
  32. Jester King blog post about green bottles
  33. Jester King on the Sour Hour, pt 2
  34. Conversation between Pierre Tilquin and Dave Janssen, 24-Oct-2015
  35. Kemp, O., Hofmann, S., Braumann, I., Jensen, S., Fenton, A., and Oladokun, O. (2021) Changes in key hop-derived compounds and their impact on perceived dry-hop flavour in beers after storage at cold and ambient temperature. J. Inst. Brew., https://doi.org/10.1002/jib.667.
  36. Matthew Sheehan, Austin Montgomery, Julie M. Goddard & Gavin L. Sacks (15 Oct 2024): Lactic and Acetic Acids in Sour Beers Promote Corrosion During Aluminum Beverage Can Storage, Journal of the American Society of Brewing Chemists, DOI: 10.1080/03610470.2024.2395677.
  37. Pineton de Chambrun G, Body-Malapel M, Frey-Wagner I, Djouina M, Deknuydt F, Atrott K, Esquerre N, Altare F, Neut C, Arrieta MC, Kanneganti TD, Rogler G, Colombel JF, Cortot A, Desreumaux P, Vignal C. Aluminum enhances inflammation and decreases mucosal healing in experimental colitis in mice. Mucosal Immunol. 2014 May;7(3):589-601. doi: 10.1038/mi.2013.78. Epub 2013 Oct 16. PMID: 24129165; PMCID: PMC3998638.
  38. Wudi Hao, Chenyu Hao, Chengrong Wu, Yuqing Xu, Cuihong Jin,Aluminum induced intestinal dysfunction via mechanical, immune, chemical and biological barriers, Chemosphere. Volume 288, Part 2, 2022, 132556, ISSN 0045-6535. https://doi.org/10.1016/j.chemosphere.2021.132556.
  39. Conversation with Chris Anderson on MTF. 9/22/2016.
  40. Rowan Chadwick. Milk The Funk Facebook group thread on bottle cleaners for small brewers. 04/25/2018.