Difference between revisions of "Wild Yeast Isolation"
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* [http://bootlegbiology.com/diy/isolating-yeast/ Bootleg Biology's] guide to streaking agar plates to isolate the captured yeast cultures. | * [http://bootlegbiology.com/diy/isolating-yeast/ Bootleg Biology's] guide to streaking agar plates to isolate the captured yeast cultures. | ||
* [http://suigenerisbrewing.blogspot.ca/2014/08/your-home-yeast-lab-made-easy-streak.html Bryan Heit's] video guide on how to streak agar plates. | * [http://suigenerisbrewing.blogspot.ca/2014/08/your-home-yeast-lab-made-easy-streak.html Bryan Heit's] video guide on how to streak agar plates. | ||
| − | * [http://suigenerisbrewing.blogspot.ca/2014/12/two-new-videos.html Bryan Heit's] video guide on streaking plates and further isolating yeast. | + | * [http://suigenerisbrewing.blogspot.ca/2014/12/two-new-videos.html Bryan Heit's] video guide on streaking plates and further isolating yeast. |
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| + | ===Identifying=== | ||
| + | * [http://suigenerisbrewing.blogspot.ca/2014/12/new-video.html Bryan Heit's] video guide on how to visually identify usable yeasts versus potential pathogens and molds. | ||
==Growing Without Isolation== | ==Growing Without Isolation== | ||
Revision as of 10:46, 21 May 2015
Wild yeast isolation, sometimes known as yeast wrangling, refers to the process of catching wild yeast, and isolating it with agar plates as a pure culture. This article will also contain information for growing up a captured mixed culture in wort rather than isolating pure cultures.
- Special thanks goes out to Bryan Heit from Sui Generis blog and Jeff Mello from Bootleg Biology for providing the information in this article.
Contents
Isolating Pure Cultures
Preparation
- Bryan Heit's video guide to setting up a clean workspace, building and using an alcohol lamp, and aseptic techniques.
- Bootleg Biology's guide on creating agar plates using wort, agar powder, and yeast nutrient.
- Bryan Heit's guide to more types of agar plates, and video tutorial.
Catching
- Bootleg Biology defines three methods of catching a wild yeast.
- Bryan Heit's video tutorial for catching wild yeast from fruit or from the air.
Isolating
- Bootleg Biology's guide to streaking agar plates to isolate the captured yeast cultures.
- Bryan Heit's video guide on how to streak agar plates.
- Bryan Heit's video guide on streaking plates and further isolating yeast.
Identifying
- Bryan Heit's video guide on how to visually identify usable yeasts versus potential pathogens and molds.
Growing Without Isolation
(To do)
See Also
External Resources
- Biology provides a full toolkit and materials for yeast isolation.
- Sui Generis blog by Bryan Heit.
- BKYeast, How to Build a Yeast Ranch.