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Isotonic Sodium Chloride is a saline solution which can be used for storage of microbes. Depending on the species, survivability times can range. It should be kept cold just above freezing. Most '''Saccharomyces''' strains handle this storage well and can be stored for 2 years like this before needing to re-propagate and re-banking. After one year '''Lactobacillus''' and '''Pediococcus''' seem to survive moderately well depending on the strain. Brettanomyces however seems to have a harder time maintaining good vitality. Please refer to [https://www.facebook.com/groups/MilkTheFunk/permalink/1888015027893369/ Justin Amaral's post] for information about this.

The solution for Isotonic Sodium Chloride is quite simple. You'll need 9 grams of lab grade Sodium Chloride to 1000 mL of distilled/de-ionized water, autoclave'dautoclaved.