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Tetrahydropyridine

561 bytes added, 16:43, 2 November 2017
update "What We Known'; with the alkaline strip technique
# What are the unidentified transient forms of THP, and do they apply to beer or just wine?
# Does the release of lysine from yeast autolysis when aging on trub increase THP potential (some say it does in wine and cider <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1852891514739054/ Tariq Ahmed of Revel Cider. Milk The Funk Facebook thread on THP in wines/ciders aged on lees. 10/16/2017.]</ref>)?
<nowiki>*</nowiki> Due to the specialized GC/MS equipment needed for measuring forms of THP that most labs do not have, certain answers will be difficult to obtain. Some studies have used alkaline strips as a way to smell the aroma of THP, and while not as precise as the specialized GC/MS lab equipment, could still help further the understanding of THP. These strips were prepared in the following way: knowing that mousy off-flavour has a lingering sensory impact, the technique of alkaline paper strip assessment was adapted from Heresztyn (1986a). Paper strips (Whatman No. 1, 4–5 mm × 50 mm) were prepared by soaking in NaOH (0.1M) and drying overnight at room temperature. The alkaline paper strips were then briefly dipped into cell-free samples (centrifuged) and immediately assessed for the mouse-like odour by sniffing <ref>Private correspondence with Dr. Paul Grbin by Dan Pixley. 11/2/2017.</ref>.
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