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Previous experiments on survival of ''Brettanomyces'' by Richard Preiss and myself (see [[Brettanomyces_Storage_Survival_Experiment#Preliminary_Work_on_30_Day_Storage|Preliminary Work On 30 Day Storage]]) have shown the yeast survives better in cold temperatures up to 1 month. However, in my previous experiment both isolates seemed to have survived better at room temperature after 6 months although at a very low survival rate. The 6 months evaluation of that experiment had issues and therefore was repeated here. In Richards experiment, evaluation at 6 months revealed mixed results with one strain surviving better in cold temperature and the other better at warm temperature. Therefore, optimum survival temperature may vary by strain. In this experiment only one strain was used but clearly survived better after 100 days at room temperature in unbuffered MYPG. Buffering was deleterious to survival at room temperature in both media, while buffering MYPG for cold temperature was an advantage. It has long been known that ''Brettanomyces'' survives poorly on plates and in my experience poorly on slants. This experiment indicated that Brett survival on slants can be improved by buffering MYPG. Although the survival on slants was only tested as live or dead, the yeast on buffered MYPG grew up nicely after 100 days while the yeast on MYPG was dead. Since the yeast was dead on both buffered and unbuffered plates at 100 days, it is not known if buffering was an advantage for survival on plates.