Changes

==Discussion==

The results indicate that for this strain of ''Brett'' a near equal cell density can be achieved with aerated or semi-aerated propagation while anaerobic incubation under agitation or still incubation resulted in less than half the cell density when compared to the former treatments. While the pH decreased with more aeration during propagation, it is interesting that the pH of all treatments were near equal by the end of the experiment. The cell counts reached by the aerobic and semi-aerobic treatments were much higher than reported in the literature and the time to reach maximum cell density is much shorter than previous reports for propagation of ''Brettanomyces''.

This experiment demonstrated a significantly faster growth rate than many other growth rate studies <ref name="Yakobson">[http://www.brettanomycesproject.com/dissertation/propagation-and-batch-culture-growth/propagation-results/ The Brettanomyces Project. Propagation and Batch Culture Results. Retrieved 11/05/2015.] </ref>. It has been suggested by MTF member and microbiologist [http://www.escarpmentlabs.com/ Richard Preiss] that propagation times vary widely among ''Brett'' strains, and that the selected strain most likely exhibits faster reproduction than other strains <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1168024059892473/?comment_id=1168034189891460&offset=0&total_comments=20&comment_tracking=%7B%22tn%22%3A%22R4%22%7D Comments by Richard Preiss on MTF regarding Mark Trent's Brett growth experiment. 10/23/2015.]</ref>. Additionally, Trent's experiment did not demonstrate the same "second lag phase" as shown in Chad Yakobson's "The Brettanomyces Project" <ref name="Yakobson"></ref>, including for the lower cell count treatment in the second experiment. Richard Preiss hypothesizes that one possibility to explain this is that the cells had a two step growth process applied in preparation for the experiment, and that this prior environment allows the ''Brett'' cells to adapt their metabolism to malt sugars. Preiss reports seeing something similar when growing ''Brett''; when first grown in YPD, then in ~50 mL of wort, the culture then grows to maximum cell density within 24-72 hours (depending on strain). Preiss also notes that not all strains of ''Brett'' exhibit the "second lag phase", and that the chosen ''Brett'' strain may be one of these strains <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1168024059892473/?comment_id=1168034189891460&reply_comment_id=1169578973070315&total_comments=2&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Comments by Richard Preiss on MTF regarding Mark Trent's Brett growth experiment. 10/23/2015.]</ref>.

==Conclusion==