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Lactobacillus

265 bytes added, 14:22, 30 September 2015
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====Storage====
For dried ''Lactobacillus'', such as probiotics or [[Dry Yeast for Sour Ales BlackManYeast]] products, [http://suigenerisbrewing.blogspot.com/ Bryan Heit's] lab studies have shown that they can lose viability ~80 times faster at room temperature than when stored at refrigeration temperatures. Therefore, it is recommended to store dried ''Lacto'' at refrigeration temperatures.
Liquid cultures become stressed by two factors: storage in an acidic environment, and storage without sugar<ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1154970097864536/?comment_id=1156656834362529&offset=0&total_comments=12&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with Bryan Heit on stress factors in storing Lacto. 09/28/2015.]</ref>. Sugar storage creates more acid as the ''Lacto'' ferments it, so it may not be ideal unless the ''Lacto'' is continually fed. Ideally, liquid cultures of ''Lacto'' should be stored frozen with 20% glycerol, or refrigerated as slants with water or mineral oil. Also, there is anecdotal evidence that certain species may survive better at room temperature. Bryan hypothesizes that stable temperatures may be more important than storing at an "ideal" temperature <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1067614393266774/ Conversation with Bryan Heit on Milk The Funk. 05/04/2015.]</ref>.
A practical option for brewers without a laboratory is to store the liquid culture with a few grams of a buffering chemical such as potassium phosphate, calcium sulfate (gypsum), or calcium hydroxide (pickling lime). The exact amounts should be adjusted to reach a pH of about 4.0 for the entire solution (begin with 1 or 2 grams per liter, and adjust as needed) <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1095449350483278/?comment_id=1095492120479001&offset=0&total_comments=23&comment_tracking=%7B%22tn%22%3A%22R6%22%7D Conversation with Adi Hastings on MTF. 6/20/2015.]</ref>.

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