Spontaneous Fermentation

Spontaneous Fermentation, for the purposes of this article, refers to the fermentation of wort by inoculating it in a coolship where the wort is left exposed to the air and allowed to cool naturally over night. Wild yeast and bacteria are introduced into the wort as it cools. It is the traditional method of brewing Lambic ^[1].

Defining Spontaneous Fermentation

(in progress) In the most romanticized view of the term spontaneous fermentation, the microbes which inoculate the wort in the coolship are sourced exclusively from the ambient environment outside the brewery. Scientific publications have suggested that in the case of some producers, these microbes may be resident in the brewhouse ^[2]. This is supported by the reluctance of lambic brewers to alter their facilities (remodeling, moving, painting, etc.) and the spraying of lambic on the walls of new buildings ^[3] ^[4]. The microbes responsible for spontaneous fermentation may also be derived from the oak barrels and/or foedres which are often used to hold the fermenting beer ^[5]. Many Belgian lambic producers thoroughly clean their barrels using hot water/steam, mechanical agitation, and/or burning sulfur ^[6]; however even the most rigorous cleaning does not likely fully sterilize the barrels. In the case of lambic brewers the microbes resident in barrels are spontaneous in origin, having been derived from years to decades of use in the brewery without any exposure to pitched cultures. The barrels may serve as a concentrating mechanism for the desired cultures.

A spontaneous fermentation may also be achieved by inoculating small amounts of wort and growing up the spontaneously inoculated microbes to check for suitability. This is common in homebrew production ^[7] and allows for screening of the microbes to remove wild cultures with aggressive off flavors and/or mold. This is not unlike the potential of used oak barrels, where well performing barrels may be kept to inoculate subsequent batches while poorly performing barrels may be discarded and removed from the brewery. As different microbes survive and thrive in different environments, barrels or pre-screened and grown starters cannot provide a complete profile of the microbes present in traditional spontaneous fermentation beers. However a combination of a coolship to inoculate the wort with ambient/brewhouse resident microbes combined with a form of pre-screening such as barrel re-use and/or spontaneous starters may provide the full microbiota present in traditional spontaneously fermented products.

Brewing Methods

(in progress)

The traditional production of spontaneously fermented beer employs three main ---points---: the production of a dextrinous wort, high hopping rates with aged hops, and inoculation of the wort with a coolship. Not all breweries producing spontaneously fermented beer employ these three techniques, but they are generally common among producers.

A dextrinous wort may be produced by different mashing procedures. The most traditional method of achieving this is through a turbid mash. With this mashing technique, unconverted starchy wort (which turbid in appearance)is pulled from the mash and heated to denature enzymes. These pulled runnings are then replaced by infusions of hot water as the mash is carried through a series of steps for conversion of the remaining grains. The starchy wort from the early 'turbid' pulls is carried to the boil with incomplete conversion, providing dextrins to sustain Brettanomyces and lactic acid bacteria in a prolonged mixed fermentation. Other methods to carry unconverted dextrins into the boil may be employed such as the addition of flour (----add source----), passing mash runnings through flaked grains (---flat tail on the brewing network---), or pulling mash runnings before full conversion without the prolonged processing of a turbid mash. Whichever technique is employed, the goals are the same - to provide starches which Saccharomyces cerevisiae and Saccharomyces pastorianus can not ferment and which can feed the diverse combination of other yeasts and bacteria present.

High hopping and aged hops

Coolship (Anglicized version of the Dutch/Flemish koelschip) is a type of fermentation vessel used in the production of beer. Traditionally, a coolship is a broad, open-top, flat vessel in which wort cools. The high surface to mass ratio allows for more efficient cooling. Contemporary usage includes any open fermentor used in the production of beer, even when using modern mechanical cooling techniques. Traditionally, coolships were constructed of wood, but later were lined with iron or copper for better thermal conductivity.

(to do) Coolship design include ref from James Howat

Homebrew 15 Gallon Coolship
Homebrew 15 Gallon Coolship
30 Barrel Coolship from Funk Factory

Microbial Succession During Fermentation

Scientific research in Belgium and the US has shown a regular general pattern to the microbial succession of spontaneous fermentation beer. ^[8] ^[9] ^[10] ^[11]. The first stage, which lasts for approximately 1 month ^[8] ^[12], is dominated by enterobacteria. Though enterobacteria contribute little in terms of gravity drop over the first month of fermentation, they may contribute aroma and flavor compounds and precursors during the initial stages of spontaneous fermentaiton ^[12].

(To Do, include link to Raj Apte)