Wild Yeast Isolation
Wild yeast isolation, sometimes known as yeast wrangling, refers to the process of catching wild yeast, and isolating it with agar plates as a pure culture. This article will also contain information for growing up a captured mixed culture in wort rather than isolating pure cultures.
- Special thanks goes out to Bryan Heit from Sui Generis blog and Jeff Mello from Bootleg Biology for providing the information in this article.
Isolating Pure Cultures
Preparation
- Bryan Heit's video guide to setting up a clean workspace, building and using an alcohol lamp, and aseptic techniques.
- Bootleg Biology's guide on creating agar plates using wort, agar powder, and yeast nutrient.
- Bryan Heit's guide to more types of agar plates, and video tutorial.
Catching
- Bootleg Biology defines three methods of catching a wild yeast.
- Bryan Heit's video tutorial for catching wild yeast from fruit or from the air.
Isolating
- Bootleg Biology's guide to streaking agar plates to isolate the captured yeast cultures.
- Bryan Heit's video guide on how to streak agar plates.
- Bryan Heit's video guide on streaking plates and further isolating yeast.
Growing Without Isolation
(To do)