Brettanomyces

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Brettanomyces

Brettanomyces, also known as brett or bretta, is a yeast that was originally thought of as a spoilage yeast. Known for it's barnyard, fecal, horsey, metallic or Band-Aid flavors, brett was unwelcome in most breweries. However, in some styles like Saison and Lambic these flavors add a layer of complexity to the beer.

Brettanomyces Metabolism

Carbohydrate Metabolism

Brettanomyces strains may possess both alpha and beta glucosidases. These enzymes allow Brettanomyces strains to break down longer chain carbohydrate molecules and to liberate glycosidically bound sugars which are unfermentable to Saccharomyces yeasts.

The cleavage of glycosides, which are sugar molecules connected to other compounds, allows Brettanomyces to liberate aroma and flavor compounds into their aroma-active and flavor-active states. Through this activity Brettanomyces strains are able to produce novel flavors and aromas from hops fruits, and fruit pits. In addition, the liberated aroma and flavor active compounds may be further processed by Brettanomyces in ester production or destruction pathways.

Ester Production and Destruction

Phenol Production

Phenols such as 4-vinylphenol (4VP, barnyard, medicinal) and 4-vinylguaiacol (4-VG, clove) can be produced in beer by the decarboxylation of hydoxycinnamic acids, which are found in malt. While both Saccharomyces and Brettanomyces strains are capable of converting hydroxycinnamic acids to their vinyl derivatives, Brettanomyces is also able to reduce these vinyl derivatives to ethyl derivatives. These vinyl derivatives have similar tastes to the ethyl derivatives but have lower flavor thresholds.

Acid Production

In the presence of oxygen, Brettanomyces strains are capable of producing acetic acid. Depending on the brewer's palate and the degree of acetic production, this can be a desirable or undesirable trait. The degree of acetic acid production varies among different brett strains. Acetic acid produced by Brettanomyces may also be used in the synthesis of acetate esters such as ethyl acetate.

Brett Strains

Isolates

In cooperation with Funk Factory

Common Name Species Name Synonym (Strain) Name Lab/Package Flavor/Aroma Source Note
Anomala Dekkera anomala B. intermedius ECY-04 strong ester profile with some light funk and acidity beer - Adelaide, Australia
Anomalus Dekkera anomala B. anomalus Wyeast bottled stout - Burton on Trent, England
Bruxellensis Dekkera bruxellensis B. bruxellensis BSI Same as White Labs Pro-Brewers only.
Bruxellensis Dekkera bruxellensis B. bruxellensis WLP650 Barnyard Not the same as WY's Brux
Bruxellensis Dekkera bruxellensis B. bruxellensis Wyeast 5112 "sweaty horse blanket" Not the same as WL's Brux
Bruxellensis Dekkera bruxellensis B. bruxellensis ECY-05 funky with barnyard notes accompanied by some fruit isolated from Belgian stout
Bruxellensis Dekkera bruxellensis B. bruxellensis RVA 502 A medium-intensity Brettanomyces yeast strain. Will add a bit of funk when added during the secondary. Typically used in Belgian-style beers, especially lambic. A famous Trappist brewery produces its unique beer with this yeast during secondary fermentation. RVA Yeast Labs
Bruxellensis Dekkera bruxellensis B. bruxellensis GB144 Produces a sweet, slightly fruity profile with just a hint of barnyard and spicy phenolics GigaYeast
Claussenii Dekkera anomala B. claussenii BSI Same as White Labs Pro-Brewers only.
Claussenii Dekkera anomala B. claussenii WLP645 Fruity, pineapple
Claussenii Dekkera anomala B. claussenii RVA 501 A low-intensity strain. Contributions from this strain are mostly aromas of pineapple and fruit. This strain prefers higher temperatures (85º F), but will produce nice aroma and subtle flavor at normal ale fermentation termperatures (68-72º F). RVA Yeast Labs
CMY1 Dekkera bruxellensis B. bruxellensis CMY1 BSI Chad Yakobson's mutation of BSI Drie
Custersianus Dekkera custersiana B. custersianus ECY-19 light fruit and hay Bantu beer brewery, South Africa
Drie Dekkera bruxellensis B. drei BSI "highly aromatic" Isolate from Drie Fonteinen; Pro-Brewers only.
Farmhouse ? B. fantome ECY-03 Fruity and funky profile with some acidity gradually increasing over time. Aeration has more of a muted effect Isolate from Fantome
Lambicus Dekkera bruxellensis B. lambicus BSI Same as White Labs Pro-Brewers only.
Lambicus Dekkera bruxellensis B. lambicus WLP653 Horsey, Smoky, Spicy Different from WY's "lambicus"
Lambicus Dekkera bruxellensis B. lambicus Wyeast 5526 Pie-cherry Different from WL's "lambicus"
Lambicus Dekkera bruxellensis B. lambicus RVA 503 High-intensity “Brett” strain. Very spicey with “smoky” and “horseblanket” flavors and aromas. This strain is used mostly in Lambics and Flanders sour beers. RVA Yeast Labs
Naardenensis Dekkera naardenensis B. naardenensis ECY-30 strawberry, honey, ripe fruit with a tart, citrusy acidity after 6mo of aging Isolated from Dr. Pepper
Nanus Eeniella nana B. nanus ECY-24 spicy, saison-like profile bottled beer - Kalmar, Sweden
Trois Dekkera bruxellensis B. trois WLP644 Mango, Pineapple Isolate from Drie Fonteinen, DISCOVERED NOT TO BE BRETT ON 12/10/2014

Smaller Labs

Mfg Taxonomy Notes
BKYeast Brett X1 Suspected Brettanomyces Anomalus
BKYeast Brett C1 Isolate from Cantillon Iris
BKYeast Brett C2 Isolate from Cantillon Iris
BKYeast Brett C3 Isolate from Cantillon Iris
DCYeast DCY01
Saccharolicious Brett I
Saccharolicious Brett II originates from Brasserie à Vapeur in Pipaix, Belgium, and was isolated from a bottle of Cochonne

Brett Blends

Manufacturer Common Name Notes
East Coast Yeast ECY34 Twelve (12) different isolates of Brettanomyces exhibiting high production of barnyard "funk" and esters. Dryness, ripe fruit, and acidity will be encountered over a period of months and over time (>1 yr), may display gueuze-like qualities in complexity. Contains various isolates from lambic-producers, B. bruxellensis, B. anomala, B. lambicus, and B. naardenensis. For those who want the most from Brett yeast, whether a 100% Brett fermentation is desired or adding to secondary aging projects. Suggested fermentation temperature: 60-74 F. Attenuation high.
The Yeast Bay Beersel not overly funky but there is a sweaty note hanging behind lemon and citrus fruits, nice blend of subtle funk and citrus/fruit
The Yeast Bay Brussels similar to Beersel but with more funk in aroma and less fruit, complex barnyard character
The Yeast Bay Lochristi Smells of Iris C2, probably the same, subtle blend with some delicate fruit, strawberry
Omega Yeast Labs Brett Blend #1 (Where Da Funk?) Trois and ECY-03b blend; well suited for 100% fermentation; ferments dry and not funky or acidic
Omega Yeast Labs Brett Blend #2 (Bit O' Funk) Trois, ECY-03b, and bruxellensis blend; moderate funk, pitch in secondary and leave for extended aging (3+ months)
Omega Yeast Labs Brett Blend #3 (Bring On Da Funk) Trois, ECY-03b, bruxellensis, claussenii, lambicus, custersianus, and naardenensis blend; funky and fruity given time, acid production increases with exposure to oxygen over time