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[[File:Pedio.jpg|thumb|200px|right|[https://www.instagram.com/wildandsour/ Pediococcus - picture taken by Per Karlsson]]]
'''Pediococcus''' (often referred to by brewers as "Pedio") are Gram-positive lactic acid bacteria (LAB) used in the production of Belgian style beers where additional acidity is desirable. They are native to plant material and fruits <ref name="ucdavis">[http://wineserver.ucdavis.edu/industry/enology/winemicro/winebacteria/pediococcus_damnosus.html Viticulture & Enology. UC Davis website. Pedioccous damnosus. Retreived Retrieved 07/28/2015.]</ref>, and often found in [[spontaneously fermented ]] beer as the primary source of lactic acid production (with ''P. damnosus'' being the only species identified in lambic[[Lambic]]) <ref>[http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0095384 The Microbial Diversity of Traditional Spontaneously Fermented Lambic Beer. Freek Spitaels, Anneleen D. Wieme, Maarten Janssens, Maarten Aerts, Heide-Marie Daniel, Anita Van Landschoot, Luc De Vuyst, Peter Vandamme. April 18, 2014.]</ref><ref>[[Scientific_Publications#Lambic_and_Spontaneous_Fermentation|Multiple Scientific publications linked on MTF.]]</ref>. It is also seen as a major source of beer contamination in commercial breweries due to its ability to adapt to and survive in beer. The ability to grow in beer is strain dependent rather than species dependent, however genetic differences indicate that ''P. damnosus'' and ''P. claussenii'' are better adapted to surviving in beer than ''P. pentosaceus'' <ref name="Snauwaert">[http://www.biomedcentral.com/content/pdf/s12864-015-1438-z.pdf Comparative genome analysis of Pediococcus damnosus LMG 28219, a strain well-adapted to the beer environment. Isabel Snauwaert, Pieter Stragier, Luc De Vuyst and Peter Vandamme. 2015.]</ref>. Like many bacteria, Pediococci have the ability to [https://en.wikipedia.org/wiki/Horizontal_gene_transfer transfer genes horizontally] without reproduction <ref name="Snauwaert"></ref>. They are generally considered to be facultative anaerobes, which means they grow anaerobically but can also grow in the presence of oxygen <ref>[http://textbookofbacteriology.net/lactics.html Lactic Acid Bacteria. Todar's Online Texbook of Bacteriology. Kenneth Todar, PhD. Pg 1. Retrieved 08/09/2015.]</ref>. Some species/strains (including individual strains of ''P. damnosus'') can have their growth and acid production inhibited by oxygen <ref name="NAKAGAWA">[https://www.jstage.jst.go.jp/article/jgam1955/5/3/5_3_95/_article TAXONOMIC STUDIES ON THE GENUS PEDIOCOCCUS. ATSUSHI NAKAGAWA, KAKUO KITAHARA. 1959.]</ref>, while some will have better growth and produce more acid in the presence of oxygen (microaerophilic) <ref>[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC357257/ THE NUTRITION AND PHYSIOLOGY OF THE GENUS PEDIOCOCCUS. Erling M. Jensen and Harry W. Seeley. 1954.]</ref><ref>[http://www.microbialcellfactories.com/content/8/1/3#B5 Pediocins: The bacteriocins of Pediococci. Sources, production, properties and applications. Maria Papagianni and Sofia Anastasiadou. 2009.]</ref>. Strains found in beer are hop tolerant <ref>[http://www.biomedcentral.com/1471-2164/16/267 Comparative genome analysis of Pediococcus damnosus LMG 28219, a strain well-adapted to the beer environment. Isabel Snauwaert, Pieter Stragier, Luc De Vuyst and Peter Vandamme. April 2015.]</ref>. Due to their continued metabolism of longer chain polysaccharides, acid production will increase with storage time. ''Pediococcus'' can form a [[pellicle]].
''Pediococcus'' may also cause “ropiness” (also called a "sick beer") due to the production of exopolysaccharides when exposed to a fresh sugar source. "Ropy" or "sick" beer is more viscous and, in extreme circumstances, can form strands. Sickness effects mostly the mouthfeel and appearance of beer, and may have no influence on the flavor. It is considered a temporary flaw in sour beer. Some brewers, including Vinnie Cilurzo from Russian River Brewing and some Belgian lambic producers, claim that after the ropiness goes away (generally in 3-6 months <ref name="ropy_time"></ref>) it produces a deeper acidity and mouthfeel, and is viewed as a positive process in the production of sour beer <ref>[http://www.xxlbrewing.com/hb/sour_beer/img_09.html Cilurzo, Vinnie. AHA Sour Beer presentation. 2007.]</ref>. For other brewers, ropy beer is seen as a nuisance due to the beer needing to be aged for a longer period of time, especially when it occurs shortly after bottling. ''Pediococcus'' species can also produce diacetyl with extended storage time <ref name="Garcia-Garcia" />. [[Brettanomyces]] can break down exopolysaccharides and diacetyl produced by ''Pediococcus'' and the two are often used together.
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| [[Bootleg Biology]] || Sour Weapon (Pediococcus pentosaceus Blend) || P. pentosaceus blend || Perfect for acidifying unhopped wort quickly for kettle or “quick” sours. At 98F, it’s capable of achieving a pH of 3.3 within 18 hours. At 84F, it can reach a pH of 3.5 within 24 hours. With more time, a terminal ph pH of 3.1 may be reached. ''P. pentosaceus'' can also be used for long-term sours. It is capable of growing and producing lactic acid in worts with IBUs as high as 30, though it is recommended for unhopped worts as IBUs over 10 may prevent significant quick souring. This culture may produce antimicrobials called bacteriocins or pediocins. These can inhibit and kill similar species of bacteria like Lactobacillus and other Pediococcus species in mixed-culture fermentations. Read Bootleg Biology's [https://www.facebook.com/BootlegBiology/photos/a.148869931970401.1073741829.124634287727299/465185997005458/?type=1&theater Facebook post] regarding bacteriocins for more info. No signs of ropiness (exopolysaccharides) have occurred in testing <ref>[http://bootlegbiology.com/product/sour-weapon-pediococcus-pentosaceus-blend/ Bootleg Biology website. Retrieved 05/06/2016.]</ref>. It is still unknown how hops will affect souring in a long term scenario. Bootleg Biology is still researching long term effects and awaiting peoples feedback as of 5/23/2016.
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| [[Inland Island Brewing & Consulting|Inland Island Yeast Laboratories]] || INISBC-998 || damnosus || Gram positive cocci that produces lactic acid. Also produces diacetyl and several proteins that may cause a "rope" to form in the beer. Rope will disappear with time. Oxygen and hop sensitive. 75-90°F Temperature Range. '''No longer available.'''