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Bottle dregs can also be used a primary fermenter. It is generally advised that mixing different bottle dregs together will provide a greater diversity and thus better results, but using a single bottle may prove to be an educational experience as well. Step the starter up to a 2 liter volume, incubate for another 7 days as above, then pitch into a ~20 liter batch. Alternatively, Jeffrey Crane describes a method of using 1 gallon of wort, which can easily be produced on a normal brew day from a larger batch, to create sour beer from bottle dregs. Essentially this process involves transferring 1 gallon of at wort any time during the boil when there are less than 7-10 IBU's. That 1 gallon of wort is then used to pitch the sour beer dregs starter into.<ref>[http://www.homebrewtalk.com/f127/easy-way-make-sour-beers-1-gal-wort-dregs-189748/ Jeffrey Crane] describes how to make 1 gallon sour beers from bottle dregs.</ref> The brewer has the option of allowing the 1 gallon of sour beer to age on it's own, or it can be used as a starter itself for a larger batch. Pitch a smaller amount of the dregs starter for 1 gallon batches (~200 mL).
===Sour worting and , co-pitching, and staggered pitching===
If there was no signs of ''Saccharomyces'' fermentation in the dregs starter then another option is to pitch the starter of dregs into the wort, and allow for a day or two to pass before pitching a fresh pitch of ''Saccharomyces''. This has the benefit of allowing the lactic acid bacteria in the dregs starter to sour the wort before competing with ''Saccharomyces'' for sugar. See [[Sour_Worting#Souring_in_the_Primary_Fermenter|souring in the primary fermenter]] for more information on this technique. Co-pitching the dregs with a fresh pitch of ''Saccharomyces'' at the same time will often achieve a less acidic final product depending on how aggressive the bottle dreg cultures are. Pitching a sour culture after primary fermentation has been reported to preesrve more yeast flavor, or allowing the ''Brettanomyces'' character to be more forward. See the [[Alternative_Bacteria_Sources#MTF_.22Reverse_Kettle_Sour.22|"MTF Reverse Kettle Sour"]] for an example.