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updated low pH fermentation issues, and link to Tonsmeire's new article
Deciding whether or not to boil the wort can also depend on whether or not there was a considerable amount of alcohol produced, which commonly happens when the wort is contaminated with yeast. Pure cultures of ''Lactobacillus'' do not show typical signs of fermentation that we are used to seeing with yeast fermentations, such as forming a krausen, producing a lot of CO2, or fermenting wort more than ~1.005 gravity points (see [[100% Lactobacillus Fermentation]]). If a yeast contamination produces a significant amount of alcohol during the souring process, then this presents a problem when it comes to boiling. Although 100% pure ethanol boils at 173.1°F/78.4°C <ref name="boiling_of_alcohol">[http://chemistry.about.com/od/moleculecompoundfacts/f/What-Is-The-Boiling-Point-Of-Alcohol.htm Boiling temperature of ethanol]</ref>, the lower the concentration of ethanol in the wort (technically beer at this point if it has been fully attenuated by yeast), the higher the temperature required for boiling off the ethanol. For example, at 5% ABV it takes approximately 197°F/92°C for the ethanol to boil <ref>[http://www.clawhammersupply.com/blogs/moonshine-still-blog/12243869-making-moonshine-still-temperature Making Moonshine: Still Temperature. Retrieved 01/11/2016.]</ref><ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1212116758816536/?comment_id=1212140998814112&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with Russell Carpenter on MTF. 01/11/2016.]</ref>. Time is also required to boil off the ethanol, so this may not be as big of a concern as it first appears (''warning: vaporized ethanol is highly flammable)''. Another and perhaps more important consideration is that boiling and high heat pasteurization temperatures can have a negative effect on the flavor of fermented beer. Beer already has anti-bacterial properties, such as low pH, presence of alcohol, and hops (although hops may not be present in wort being soured), so higher pasteurization temperatures aren't necessarily required for beer. For these reasons, the beer industry commonly heat pasteurizes beer at 140°F/60°C for 15 minutes, and this is also adequate for pasteurizing soured beer <ref>[http://www.sciencedirect.com/science/article/pii/S0023643806002854#bib16 A suitable model of microbial survival curves for beer pasteurization. Sencer Buzrul. 2006.]</ref>. In the case of an accidental yeast contamination during the souring process, another option is to simply dump the batch and start again with a pure culture of ''Lactobacillus''. Boiling soured wort that hasn't had an accidental yeast contamination (and thus still has a high specific gravity) probably has less of an effect on the flavor than it does on fully fermented beer. Other than lactic acid, the flavor components that different strains of ''Lactobacillus'' produce are not well defined, so it will be difficult to determine if boiling soured wort will have a negative impact. However, brewers who boil kettle soured wort don't often report that the boiling causes flavor issues <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1464590560235820/?comment_id=1465040723524137&reply_comment_id=1465146946846848&comment_tracking=%7B%22tn%22%3A%22R4%22%7D Conversation with Bryan from Sui Generis blog on MTF regarding boiling soured wort. 11/10/2016.]</ref>.
Once the soured wort is boiled or heat pasteurized, it can be safely added to the primary fermenting vessel without worries of future infections. The wort is aerated as normal, and brewer's yeast, or ''[[Brettanomyces]]'' yeast is then pitched into the wort as normal (usually brewer's yeast is used if infection of cold side equipment is a concern). At a pH of 3.4 or lower, the acidity of the wort can reportedly effect the fermentation of some strains of brewer's yeast <ref name="low ph">[http://www.homebrewtalk.com/f127/no-hop-berliner-weisse-415067/index3.html#post5280971 Michael Tonsmeire on HBT]</ref><ref>[http://www.themadfermentationist.com/2016/12/quick-sour-then-what-acid-tolerance-of.html "Quick Sour, then what? Acid Tolerance of Brewer’s Yeast." The Mad Fermentationist blog. Michael Tonsmeire. 12/13/2016. Retrieved 12/14/2016.]</ref>. It is recommended to pitch a healthy starter of yeast, possibly with a higher cell count than normal. In the case of using dry yeast, re-hydrating as per the manufacturer's recommendations and using a yeast nutrient like Go-Ferm is recommended. Many yeast strains have been successfully used by MTF members to ferment pre-acidified wort: US05, S04, WY1098/WLP007/OYL-006, Belle Saison, Sacch Trois, Bret brux, B. clausenii, B. custersianus, Bret Drie (BSI), WY3711, and WY3726 to name a few (see reference) <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1105185972842949/ Conversation on MTF about using specific yeast strains in acidic wort. 7/6/2015.]</ref>. [http://www.themadfermentationist.com/2016/12/quick-sour-then-what-acid-tolerance-of.html Michael Tonsmeire] has shown results that imply that English yeast strains might attenuate slightly more and give better flavor results than other strains; Richard Preiss from Escarpment Labs expressed similar observations <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1509509862410556/?comment_id=1509520015742874&comment_tracking=%7B%22tn%22%3A%22R4%22%7D MTF conversation with Richard Preiss about English ale strains and pH tolerance. 12/13/2016.]</ref>. Brewers who are having difficulty fully fermenting pre-acidified wort can try growing their yeast in the soured wort (pasteurize the soured wort first if needed) with yeast nutrients (Fermaid K + DAP, for example). This assumes that the wort still has a lot of sugar left over after souring (if not, DME can be added). See [[SaccharomycesPackaging#Fermentation_Under_Low_pH_ConditionsAcid_Shock_Starters|Fermenting in low pH conditionsAcid Shock Starters]] for more information.
===Souring in the Primary Fermenter===