Changes

Survival of yeast in 4 different liquid media preparations was tested in cold and room temperature conditions. Media were; wort, buffered wort, MYPG, and buffered MYPG. In addition yeast was stored cold on solid media plates and slants containing buffered MYPG or MYPG. For liquid media, the yeast survived better at room temperature conditions compared to refrigerated conditions with the exception of survival in buffered MYPG cold treatment which was not significantly different from survival in buffered wort room or in buffered MYPG room (Fig. 2). Yeast stored on plates were dead on both media as was the yeast stored on MYPG slants while the yeast stored on buffered MYPG "slants" was viable in both replicates (Fig. 34).